Invitrogen

ASK1 Recombinant Rabbit Monoclonal Antibody (SU36-06)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-42430

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1:50-1:100
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SU36-06

Immunogen

Synthetic peptide within N-terminal human ASK1
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911571

Product Specific Information

Positive Control: 293 cell lysates, A549, Hela, MCF-7, human lung carcinoma tissue, human pancreas tissue, mouse pancreas tissue, human breastcarcinoma tissue.

Subcellular Location: Endoplasmic reticulum, Cytoplasm.

Target Information

MAP3K5 (ASK1) is a serine/threonine kinase that is abundantly expressed in human heart and pancreas and are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activates c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death and a catalytically inactive form of ASK1 inhibited TNF-a-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: apoptosis signal regulating kinase 1; Apoptosis signal-regulating kinase 1; ASK 1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; mitogen activated protein kinase kinase kinase 5; Mitogen-activated protein kinase kinase kinase 5; RP3-325F22.4

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Gene Aliases: 7420452D20Rik; ASK; ASK1; MAP3K5; MAPKKK5; MEKK5

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UniProt ID: (Human) Q99683, (Mouse) O35099

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Entrez Gene ID: (Human) 4217, (Mouse) 26408

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Function(s)
magnesium ion binding protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity protein binding ATP binding protein kinase binding protein phosphatase binding protein homodimerization activity nucleotide binding cysteine-type endopeptidase activator activity involved in apoptotic process kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding
Process(es)
MAPK cascade activation of MAPKK activity response to ischemia protein phosphorylation JNK cascade activation of JUN kinase activity intrinsic apoptotic signaling pathway in response to oxidative stress viral process response to endoplasmic reticulum stress p38MAPK cascade positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of JUN kinase activity innate immune response positive regulation of myoblast differentiation positive regulation of JNK cascade intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide apoptotic signaling pathway programmed necrotic cell death positive regulation of neuron death cellular response to reactive nitrogen species activation of MAPK activity positive regulation of protein phosphorylation immune system process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process regulation of cell death phosphorylation
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