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Invitrogen
ATF1 Monoclonal Antibody (7F8)
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FIGURE: 1 / 6
ATF1 Antibody (MA5-49203) in ICC/IF
Immunocytochemistry analysis of ATF1 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in ATF1 Monoclonal antibody (Product # MA5-49203) using a dilution of 2 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More
Product Details
MA5-49203
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
7F8
Immunogen
E.coli-derived human ATF1 recombinant protein (Position: M1-V271).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074861
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human ATF1 shares 91% amino acid (aa) sequence identity with mouse ATF1.
Positive Control - WB: human HepG2 whole cell, human Hela whole cell, human SW620 whole cell. IHC: human lung cancer tissue, human rectal cancer tissue, human rectal cancer tissue. ICC/IF: A431 cell. Flow: K562 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating transcription factor 1; cAMP-dependent transcription factor ATF-1; Cyclic AMP-dependent transcription factor ATF-1; Protein TREB36
Gene Aliases: ATF1; EWS-ATF1; FUS/ATF-1; TREB36
UniProt ID: (Human) P18846
Entrez Gene ID: (Human) 466
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
protein complex binding
protein heterodimerization activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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