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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • ATF6 Antibodies

          Proteintech

          ATF6 Polyclonal Antibody

          212 References
          View all (30) ATF6 antibodies

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          Datasheet
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          Datasheet
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          Cite ATF6 Polyclonal Antibody

          • Antibody Testing Data (13)
          ATF6 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          ATF6 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 13

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          ATF6 Antibody (24169-1-AP) in ICC/IF

          Immunofluorescent analysis of HeLa cells using 24169-1-AP (ATF6 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          ATF6 Antibody in Immunocytochemistry (ICC/IF)
          ATF6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATF6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATF6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATF6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATF6 Antibody in Western Blot (WB)
          ATF6 Antibody in Western Blot (WB)
          ATF6 Antibody in Western Blot (WB)
          ATF6 Antibody in Western Blot (WB)
          ATF6 Antibody in Western Blot (WB)
          ATF6 Antibody in Western Blot (WB)
          ATF6 Antibody in Western Blot (WB)
          ATF6 Antibody in Immunoprecipitation (IP)

          Product Details

          24169-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 182 publications 182 publications

          Immunohistochemistry (IHC)

          -
          View 10 publications 10 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:10-1:100
          View 14 publications 14 publications

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication

          Not applicable (N/A)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Non-human primate, Pig, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          ATF6 Fusion Protein Ag21456 (1-194 aa encoded by BC014969)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.27 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment. The antibody recognizes cleaved and fully glycosylated forms of ATF6.

          Immunogen sequence: MGEPAGVAG TMESPFSPGL FHRLDEDWDS ALFAELGYFT DTDELQLEAA NETYENNFDN LDFDLDLVPW ESDIWDINNQ ICTVKDIKAE PQPLSPASSS YSVSSPRSVD SYSSTQHVPE ELDLSSSSQM SPLSLYGENS NSLSSAEPLK EDKPVTGPRN KTENGLTPKK KIQVNSKPSI QPKPLLLPAA PKTQT (1-194 aa encoded by BC014969)

          Target Information

          ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It Interacts also with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Activating transcription factor 6 alpha; Activating transcription factor 6 beta; ATF 6; ATF6 alpha; ATF6-alpha; ATF6-beta; cAMP response element binding protein-related protein; cAMP response element-binding protein-related protein; cAMP responsive element binding protein-like 1; cAMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 beta; cAMP-responsive element-binding protein-like 1; Creb-related protein; Creb-rp; Cyclic AMP-dependent transcription factor ATF-6 alpha; Cyclic AMP-dependent transcription factor ATF-6 beta; DKFZp686P2194; FLJ21663; Protein G13

          View more View less

          Gene Aliases: 9130025P16Rik; 9630036G24; AA617266; AA789574; ACHM7; ATF6; ATF6A; Atf6alpha; ATF6B; ATF6beta; CREB-RP; CREBL1; ESTM49; G13

          View more View less

          UniProt ID: (Human) P18850, (Human) Q99941, (Rat) G3V909, (Mouse) F6VAN0

          View more View less

          Entrez Gene ID: (Human) 22926, (Human) 1388, (Rat) 304962, (Mouse) 226641, (Mouse) 12915, (Rat) 406169

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding ubiquitin protein ligase binding cAMP response element binding identical protein binding protein heterodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding DNA binding
          Process(es)
          eye development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein folding response to stress positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response signal transduction visual perception endoplasmic reticulum unfolded protein response ATF6-mediated unfolded protein response positive regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress regulation of transcription, DNA-templated response to unfolded protein positive regulation of transcription from RNA polymerase II promoter
          It has to be done as per old AB suggested Products section.
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