OriGene

ATG3 Monoclonal Antibody (OTI3H2), TrueMAB™

View all (37) ATG3 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

CF503346

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

1:100
-
Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI3H2

Immunogen

Full length human recombinant protein of human ATG3 produced in HEK293T cell.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Autophagy is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2610016C12Rik; APG3-like; ATG3 autophagy related 3 homolog; autophagy-related 3; Autophagy-related protein 3; DKFZp564M1178; FLJ22125; hApg3; MGC15201; Preconditioning-inducible gene 1 protein; Protein PC3-96; Ubiquitin-like-conjugating enzyme ATG3

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Gene Aliases: 2610016C12Rik; APG3; APG3-LIKE; APG3L; ATG3; Atg3l; PC3-96; PIG-1; Pig1

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UniProt ID: (Human) Q9NT62, (Rat) Q6AZ50, (Mouse) Q9CPX6

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Entrez Gene ID: (Human) 64422, (Dog) 478564, (Rat) 171415, (Mouse) 67841

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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