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ATG4A Antibody (MA5-37669) in ICC/IF
Immunocytochemistry/Immunofluorescent analysis of ATG4A in 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells using ATG4A Monoclonal Antibody (Product # MA5-37669) at a 1:25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red).The nuclear counter stain is DAPI (blue).
Product Details
MA5-37669
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
1458CT808.66.25.69
Immunogen
ATG4A recombinant protein
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897594
Target Information
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG4 autophagy 4 homolog A; ATG4 autophagy related 4 homolog A; AUT-like 2 cysteine endopeptidase; AUT-like 2, cysteine endopeptidase; autophagin 2; Autophagin-2; autophagy related 4A, cysteine peptidase; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; Cysteine protease ATG4A; hAPG4A; HsAPG4A; MGC107179
Gene Aliases: APG4A; ATG4A; AUTL2
UniProt ID: (Human) Q8WYN0
Entrez Gene ID: (Human) 115201
Performance Guarantee
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