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ATOH1 Polyclonal Antibody

ATOH1 Antibody in Immunocytochemistry (ICC/IF)

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ATOH1 Antibody (21215-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 21215-1-AP (ATOH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ATOH1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Product Details

21215-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:250-1:1,000

View 12 publications 12 publications

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ATOH1 Fusion Protein Ag15615 (1-354 aa encoded by BC069594)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.34 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSRLLHAEE WAEVKELGDH HRQPQPHHLP QPPPPPQPPA TLQAREHPVY PPELSLLDST DPRAWLAPTL QGICTARAAQ YLLHSPELGA SEAAAPRDEV DGRGELVRRS SGGASSSKSP GPVKVREQLC KLKGGVVVDE LGCSRQRAPS SKQVNGVQKQ RRLAANARER RRMHGLNHAF DQLRNVIPSF NNDKKLSKYE TLQMAQIYIN ALSELLQTPS GGEQPPPPPA SCKSDHHHLR TAASYEGGAG NATAAGAQQA SGGSQRPTPP GSCRTRFSAP ASAGGYSVQL DALHFSTFED SALTAMMAQK NLSPSLPGSI LQPVQEENSK TSPRSHRSDG EFSPHSHYSD SDEAS (1-354 aa encoded by BC069594)

Target Information

The Drosophila atonal gene produces a protein with basic helix loop helix (bHLH) domains that plays an essential role in the development of the Drosophila nervous system. Mammalian atonal homolog 1 (MATH-1) is a helix-loop-helix (HLH) transcription factor that is structurally homologous to the product of the Drosophila proneural gene atonal. MATH-1, so known as Atoh1, Ath1 or HATH-1, is a 351 amino acid protein with an atonal-related basic HLH domain. In mice, expression of MATH-1 takes place by embryonic day 9. 5 and initially localizes to the cranial ganglions and the dorsal part of the central nervous system. Prominent expression of MATH-1 is in the dorsal part of the central nervous system but becomes restricted to the external granular layer of the cerebellum by day 18 and is undetectable in the adult nervous system. It is suggested that MATH-1 may play a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aryl Hydrocarbon Receptor Activation Coordinates Mouse Small Intestinal Epithelial Cell Programming.

Laboratory investigation; a journal of technical methods and pathology

Zhou X,Chakraborty D,Murray IA,Coslo D,Kehs Z,Vijay A,Ton C,Desai D,Amin SG,Patterson AD,Perdew GH

Wed Feb 01 00:00:00 UTC 2023

Mapping of Notch signaling in the developing organ of Corti in common marmosets.

Frontiers in neuroanatomy

Hosoya M,Fujioka M,Okano H,Ozawa H

Sat Jul 01 00:00:00 UTC 2023

Absence of gut microbiota impairs depletion of Paneth cells but not goblet cells in germ-free Atoh1^lox/lox VilCreER^T2 mice.

American journal of physiology. Gastrointestinal and liver physiology

Hassan M,Juanola O,Huber S,Kellmann P,Zimmermann J,Lazzarini E,Ganal-Vonarburg SC,Gomez de Agüero M,Moghadamrad S

Thu Jun 01 00:00:00 UTC 2023

Shaoyao decoction restores the mucus layer in mice with DSS-induced colitis by regulating Notch signaling pathway.

Journal of ethnopharmacology

Fang YX,Liu YQ,Hu YM,Yang YY,Zhang DJ,Jiang CH,Wang JH,Zhang J

Tue May 23 00:00:00 UTC 2023

YAP1 and WWTR1 expression inversely correlates with neuroendocrine markers in Merkel cell carcinoma.

The Journal of clinical investigation

Frost TC,Gartin AK,Liu M,Cheng J,Dharaneeswaran H,Keskin DB,Wu CJ,Giobbie-Hurder A,Thakuria M,DeCaprio JA

Wed Mar 01 00:00:00 UTC 2023

YAP1 and WWTR1 expression inversely correlates with neuroendocrine markers in Merkel cell carcinoma.

The Journal of clinical investigation

Frost TC,Gartin AK,Liu M,Cheng J,Dharaneeswaran H,Keskin DB,Wu CJ,Giobbie-Hurder A,Thakuria M,DeCaprio JA

Wed Mar 01 00:00:00 UTC 2023

The Transcription Factor Pou3f1 Sheds Light on the Development and Molecular Diversity of Glutamatergic Cerebellar Nuclear Neurons in the Mouse.

Frontiers in molecular neuroscience

Wu JPH,Yeung J,Rahimi-Balaei M,Wu SR,Zoghbi H,Goldowitz D

Tue Aug 09 00:00:00 UTC 2022

Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammals.

eLife

Iyer AA,Hosamani I,Nguyen JD,Cai T,Singh S,McGovern MM,Beyer L,Zhang H,Jen HI,Yousaf R,Birol O,Sun JJ,Ray RS,Raphael Y,Segil N,Groves AK

Tue Nov 29 00:00:00 UTC 2022

IL-17RA-signaling in Lgr5⁺ intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment.

Immunity

Lin X,Gaudino SJ,Jang KK,Bahadur T,Singh A,Banerjee A,Beaupre M,Chu T,Wong HT,Kim CK,Kempen C,Axelrad J,Huang H,Khalid S,Shah V,Eskiocak O,Parks OB,Berisha A,McAleer JP,Good M,Hoshino M,Blumberg R,Bialkowska AB,Gaffen SL,Kolls JK,Yang VW,Beyaz S,Cadwell K,Kumar P

Tue Feb 08 00:00:00 UTC 2022

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.

Food & function

Fang J,Zhang Z,Cheng Y,Yang H,Zhang H,Xue Z,Lu S,Dong Y,Song C,Zhang X,Zhou Y

Wed Apr 20 00:00:00 UTC 2022

Rational incorporation of any unnatural amino acid into proteins by machine learning on existing experimental proofs.

Computational and structural biotechnology journal

Zhang H,Zheng Z,Dong L,Shi N,Yang Y,Chen H,Shen Y,Xia Q

Sat Sep 24 00:00:00 UTC 2022

Sufu- and Spop-mediated regulation of Gli2 is essential for the control of mammalian cochlear hair cell differentiation.

Proceedings of the National Academy of Sciences of the United States of America

Qin T,Ho CC,Wang B,Hui CC,Sham MH

Tue Oct 25 00:00:00 UTC 2022

Early development of the cochlea of the common marmoset, a non-human primate model.

Neural development

Hosoya M,Fujioka M,Okahara J,Yoshimatsu S,Okano H,Ozawa H

Sat May 07 00:00:00 UTC 2022

Addiction of Merkel cell carcinoma to MUC1-C identifies a potential new target for treatment.

Oncogene

Morimoto Y,Fushimi A,Yamashita N,Hagiwara M,Bhattacharya A,Cheng J,Frost TC,Ahmad R,Daimon T,Huang L,Hata T,Takahashi H,Yamamoto M,Suzuki Y,DeCaprio JA,Kufe D

Fri Jul 01 00:00:00 UTC 2022

Opposing effects of Wnt/β-catenin signaling on epithelial and mesenchymal cell fate in the developing cochlea.

Development (Cambridge, England)

Billings SE,Myers NM,Quiruz L,Cheng AG

Tue Jun 01 00:00:00 UTC 2021

Intestinal MYC modulates obesity-related metabolic dysfunction.

Nature metabolism

Luo Y,Yang S,Wu X,Takahashi S,Sun L,Cai J,Krausz KW,Guo X,Dias HB,Gavrilova O,Xie C,Jiang C,Liu W,Gonzalez FJ

Thu Jul 01 00:00:00 UTC 2021

Efficient stimulation of retinal regeneration from Müller glia in adult mice using combinations of proneural bHLH transcription factors.

Cell reports

Todd L,Hooper MJ,Haugan AK,Finkbeiner C,Jorstad N,Radulovich N,Wong CK,Donaldson PC,Jenkins W,Chen Q,Rieke F,Reh TA

Tue Oct 19 00:00:00 UTC 2021

Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Jenkins BR,Blaseg NA,Grifka-Walk HM,Deuling B,Swain SD,Campbell EL,Walk ST,Kominsky DJ

Tue Jun 01 00:00:00 UTC 2021

Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris.

eLife

Wilkerson BA,Zebroski HL,Finkbeiner CR,Chitsazan AD,Beach KE,Sen N,Zhang RC,Bermingham-McDonogh O

Tue May 18 00:00:00 UTC 2021

Compound sophorae decoction enhances intestinal barrier function of dextran sodium sulfate induced colitis via regulating notch signaling pathway in mice.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Wu H,Chen QY,Wang WZ,Chu S,Liu XX,Liu YJ,Tan C,Zhu F,Deng SJ,Dong YL,Yu T,Gao F,He HX,Leng XY,Fan H

Fri Jan 01 00:00:00 UTC 2021

Merkel Cell Polyomavirus T Antigens Induce Merkel Cell-Like Differentiation in GLI1-Expressing Epithelial Cells.

Cancers

Kervarrec T,Samimi M,Hesbacher S,Berthon P,Wobser M,Sallot A,Sarma B,Schweinitzer S,Gandon T,Destrieux C,Pasqualin C,Guyétant S,Touzé A,Houben R,Schrama D

Tue Jul 21 00:00:00 UTC 2020

Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis.

Nature cell biology

Park DE,Cheng J,McGrath JP,Lim MY,Cushman C,Swanson SK,Tillgren ML,Paulo JA,Gokhale PC,Florens L,Washburn MP,Trojer P,DeCaprio JA

Fri May 01 00:00:00 UTC 2020

Dual Regulatory Functions of SUFU and Targetome of GLI2 in SHH Subgroup Medulloblastoma.

Developmental cell

Yin WC,Satkunendran T,Mo R,Morrissy S,Zhang X,Huang ES,Uusküla-Reimand L,Hou H,Son JE,Liu W,Liu YC,Zhang J,Parker J,Wang X,Farooq H,Selvadurai H,Chen X,Ngan ES,Cheng SY,Dirks PB,Angers S,Wilson MD,Taylor MD,Hui CC

Mon Jan 28 00:00:00 UTC 2019

Dual Regulatory Functions of SUFU and Targetome of GLI2 in SHH Subgroup Medulloblastoma.

Developmental cell

Mon Jan 28 00:00:00 UTC 2019

Atoh1 Directs Regeneration and Functional Recovery of the Mature Mouse Vestibular System.

Cell reports

Sayyid ZN,Wang T,Chen L,Jones SM,Cheng AG

Tue Jul 09 00:00:00 UTC 2019

Generation of Vestibular Tissue-Like Organoids From Human Pluripotent Stem Cells Using the Rotary Cell Culture System.

Frontiers in cell and developmental biology

Mattei C,Lim R,Drury H,Nasr B,Li Z,Tadros MA,D'Abaco GM,Stok KS,Nayagam BA,Dottori M

Thu Oct 01 00:00:00 UTC 2020

Sox2 haploinsufficiency primes regeneration and Wnt responsiveness in the mouse cochlea.

The Journal of clinical investigation

Atkinson PJ,Dong Y,Gu S,Liu W,Najarro EH,Udagawa T,Cheng AG

Mon Apr 02 00:00:00 UTC 2018

Sox2 haploinsufficiency primes regeneration and Wnt responsiveness in the mouse cochlea.

The Journal of clinical investigation

Atkinson PJ,Dong Y,Gu S,Liu W,Najarro EH,Udagawa T,Cheng AG

Mon Apr 02 00:00:00 UTC 2018

SCF/c-KIT Signaling Increased Mucin2 Production by Maintaining Atoh1 Expression in Mucinous Colorectal Adenocarcinoma.

International journal of molecular sciences

Shen P,Yang S,Sun H,Li G,Wu B,Ji F,Sun T,Zhou D

Tue May 22 00:00:00 UTC 2018

SCF/c-KIT Signaling Increased Mucin2 Production by Maintaining Atoh1 Expression in Mucinous Colorectal Adenocarcinoma.

International journal of molecular sciences

Shen P,Yang S,Sun H,Li G,Wu B,Ji F,Sun T,Zhou D

Tue May 22 00:00:00 UTC 2018

Wls expression in the rhombic lip orchestrates the embryonic development of the mouse cerebellum.

Neuroscience

Yeung J,Goldowitz D

Fri Jun 23 00:00:00 UTC 2017

Wls expression in the rhombic lip orchestrates the embryonic development of the mouse cerebellum.

Neuroscience

Yeung J,Goldowitz D

Fri Jun 23 00:00:00 UTC 2017

Stem cells expanded from the human embryonic hindbrain stably retain regional specification and high neurogenic potency.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Tailor J,Kittappa R,Leto K,Gates M,Borel M,Paulsen O,Spitzer S,Karadottir RT,Rossi F,Falk A,Smith A

Wed Jul 24 00:00:00 UTC 2013

Stem cells expanded from the human embryonic hindbrain stably retain regional specification and high neurogenic potency.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Tailor J,Kittappa R,Leto K,Gates M,Borel M,Paulsen O,Spitzer S,Karadottir RT,Rossi F,Falk A,Smith A

Wed Jul 24 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Atonal bHLH transcription factor 1; atonal homolog 1; atonal homolog bHLH transcription factor 1; bHLHa14; Class A basic helix-loop-helix protein 14; H MATH-1; hATH1; Helix-loop-helix protein hATH-1; Helix-loop-helix protein mATH-1; mATH1; Protein atonal homolog 1; Transcription factor ATOH1

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Gene Aliases: ATH1; ATOH1; BHLHA14; HATH1; MATH-1; Math1; RGD1565171

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UniProt ID: (Human) Q92858, (Mouse) P48985

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Entrez Gene ID: (Human) 474, (Mouse) 11921, (Rat) 500156

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding chromatin DNA binding protein dimerization activity DNA binding protein binding sequence-specific DNA binding

Process(es)

neuron migration transcription from RNA polymerase II promoter Notch signaling pathway axon guidance central nervous system development cerebral cortex development inner ear morphogenesis auditory receptor cell fate specification auditory receptor cell fate determination negative regulation of apoptotic process positive regulation of auditory receptor cell differentiation positive regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development nervous system development brain development cell differentiation neuron differentiation auditory receptor cell differentiation regulation of neuron differentiation inner ear development positive regulation of inner ear receptor cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATOH1 Polyclonal Antibody

ATOH1 Antibody (21215-1-AP) in ICC/IF

Product Details

21215-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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