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Invitrogen
ATP Citrate Lyase Recombinant Rabbit Monoclonal Antibody (4D11)
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FIGURE: 1 / 14
ATP Citrate Lyase Antibody (MA5-24861) in ICC/IF
Immunofluorescence analysis of ATP Citrate Lyase was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 0.2% BSA for 45 minutes at room temperature. The cells were labeled with ATP Citrate Lyase Recombinant Rabbit Monoclonal Antibody (4D11) (Product # MA5-24861) at 1:200 dilu... View More
Product Details
MA5-24861
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4D11
Immunogen
Recombinant fusion protein of human ACLY.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2717299
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Purity is > 95% by SDS-PAGE.
Target Information
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACL; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; Citrate cleavage enzyme
Gene Aliases: A730098H14Rik; ACL; ACLY; ATPCL; AW538652; CLATP
UniProt ID: (Human) P53396, (Mouse) Q91V92, (Rat) P16638
Entrez Gene ID: (Human) 47, (Mouse) 104112, (Rat) 24159
ATP citrate synthase activity
protein binding
ATP binding
metal ion binding
cofactor binding
nucleotide binding
catalytic activity
succinate-CoA ligase (ADP-forming) activity
transferase activity
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
lyase activity
acetyl-CoA biosynthetic process
citrate metabolic process
oxaloacetate metabolic process
fatty acid biosynthetic process
cholesterol biosynthetic process
lipid biosynthetic process
positive regulation of cellular metabolic process
long-chain fatty-acyl-CoA biosynthetic process
acetyl-CoA metabolic process
lipid metabolic process
metabolic process
cellular carbohydrate metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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