Invitrogen

ATP Synthase gamma Polyclonal Antibody

ATP Synthase gamma Antibody in Western Blot (WB)

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ATP Synthase gamma Antibody (PA5-143170) in WB

Western Blot analysis of ATP Synthase gamma in Rat Skeletal Muscle lysate (35 µg protein in RIPA buffer) using ATP Synthase gamma Polyclonal Antibody (Product # PA5-143170) at 0.1 µg/mL incubated for 1 hour. Detected by chemiluminescence.

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Product Details

PA5-143170

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.3 µg/mL

Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide with sequence C-TLKDITRRLKSIKN (aa 27-40).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2933809

Product Specific Information

Additional Information: The immunizing peptide represents the N terminus of the mature protein. This antibody is expected to recognize both reported isoforms (NP_005165.1; NP_001001973.1).

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:128,000.

Target Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP synthase subunit gamma, mitochondrial; F-ATPase gamma subunit

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Entrez Gene ID: (Rat) 116550