Invitrogen

ATP synthase Monoclonal Antibody (12F4AD8AF8)

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

ATP synthase Antibody in Immunocytochemistry (ICC/IF)

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ATP synthase Antibody (43-9700) in ICC/IF

Immunofluorescence analysis of ATP5A was performed using Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ATP5A Mouse Monoclonal Antibody (Product # 43-9700) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat a... View More

ATP synthase Antibody in Flow Cytometry (Flow)

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Product Details

43-9700

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

5 µg/mL

Flow Cytometry (Flow)

1 µg/ 10^6 cells

Product Specifications

Species Reactivity

Bovine, Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

12F4AD8AF8

Immunogen

Bovine Complex V

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533547

Product Specific Information

Positive control: Bovine heart mitochondria

Target Information

Complex V, also called F1F0ATPase or ATP synthase, is responsible for ATP production in oxidative phosphorylation and can work in reverse as a proton pumping ATPase. The enzyme was thought to be localized exclusively to mitochondria. However, it has recently been identified on the plasma membrane of several cell types including hepatocytes where it functions as the HDL receptor, on endothelial cells where it may act as the angiostatin receptor, and on the surface of cancer cells. The enzyme in mammals is composed of 17 subunits, five of which make up the easily detached F1. The remainder subunits are components of two stalk domains and the proton pumping F0 part of the machinery. Two of the subunits of the F0 part are encoded on mitochondrial DNA while the other subunits are nuclear encoded. Mutations in the mitochondrial-encoded subunits of ATP synthase (Complex V) cause OXPHOS disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 6.8 kDa mitochondrial proteolipid protein; A6L; ATP synthase alpha chain, mitochondrial; ATP synthase alpha subunit; ATP synthase B chain, mitochondrial; ATP synthase c subunit; ATP synthase D chain, mitochondrial; ATP synthase e chain, mitochondrial; ATP synthase epsilon chain, mitochondrial; ATP synthase f chain, mitochondrial; ATP synthase F(0) complex subunit B1, mitochondrial; ATP synthase F(0) complex subunit C1, mitochondrial; ATP synthase F(0) complex subunit C2, mitochondrial; ATP synthase F(0) complex subunit C3, mitochondrial; ATP synthase F1 subunit alpha; ATP synthase F1 subunit beta; ATP synthase F1 subunit delta; ATP synthase F1 subunit epsilon; ATP synthase F1 subunit gamma; ATP synthase g chain, mitochondrial; ATP synthase gamma chain, mitochondrial; ATP synthase gamma-subunit gene encoding a mitochondrial protein; ATP synthase inhibitor protein; ATP synthase lipid-binding protein; ATP synthase lipid-binding protein, mitochondrial; ATP synthase membrane subunit 6.8PL; ATP synthase membrane subunit c locus 1; ATP synthase membrane subunit c locus 2; ATP synthase membrane subunit c locus 3; ATP synthase membrane subunit e; ATP synthase membrane subunit f; ATP synthase membrane subunit g; ATP synthase peripheral stalk subunit d; ATP synthase peripheral stalk subunit F6; ATP synthase peripheral stalk subunit OSCP; ATP synthase peripheral stalk-membrane subunit b; ATP synthase protein 8; ATP synthase proteolipid P1; ATP synthase proteolipid P2; ATP synthase proteolipid P3; ATP synthase proton-transporting mitochondrial F(0) complex subunit B1; ATP synthase proton-transporting mitochondrial F(0) complex subunit C1; ATP synthase proton-transporting mitochondrial F(0) complex subunit C2; ATP synthase proton-transporting mitochondrial F(0) complex subunit C3; ATP synthase subunit 9; ATP synthase subunit a; ATP synthase subunit alpha, mitochondrial; ATP synthase subunit ATP5MJ, mitochondrial; ATP synthase subunit b; ATP synthase subunit b, mitochondrial; ATP synthase subunit beta, mitochondrial; ATP synthase subunit d, mitochondrial; ATP synthase subunit delta, mitochondrial; ATP synthase subunit e, mitochondrial; ATP synthase subunit epsilon, mitochondrial; ATP synthase subunit f, mitochondrial; ATP synthase subunit g, mitochondrial; ATP synthase subunit gamma, mitochondrial; ATP synthase subunit O, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 2; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9); ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C2 (subunit 9); ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9); ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f, isoform 2; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit; DNA segment, Chr 12, Wayne State University 28, expressed; ATP synthase, H+ transporting, mitochondrial F1F0, subunit d; ATP synthase, H+ transporting, mitochondrial F1F0, subunit g; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C2 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit G; ATP synthase, mitochondrial, C subunit-3; ATP synthase-coupling factor 6, mitochondrial; ATP sythase (F1-ATPase) alpha subunit; ATPase inhibitor protein; ATPase inhibitor, mitochondrial; ATPase protein 9; ATPase subunit 9; ATPase subunit b; ATPase subunit C; ATPase subunit d; ATPase subunit e; ATPase subunit epsilon; ATPase subunit F6; ATPase subunit G; cell proliferation-inducing protein 47; coupling factor 6; epididymis secretory protein Li 271; epididymis secretory sperm binding protein Li 123m; F(0)F(1)-ATPase; F-ATPase delta subunit; F-ATPase gamma subunit; F-ATPase protein 6; F-ATPase subunit 8; F1 gamma; F1-ATPase beta-subunit; F1F0-ATP synthase g subunit; F1F0-ATP synthase, murine e subunit; F1F0-type ATP synthase subunit g; F1F0-type ATPase subunit f; F1Fo-ATP synthase complex Fo membrane domain f subunit; F1Fo-ATP synthase complex Fo membrane domain g subunit; F1Fo-ATPase synthase f subunit; H(+)-transporting two-sector ATPase; H+-ATP synthase subunit b; human ATP synthase OSCP subunit; hypothetical protein, clone:2-31; IF(1); IF1; Inhibitor of F(1)F(o)-ATPase; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase complex delta-subunit precusor; mitochondrial ATP synthase epsilon chain; mitochondrial ATP synthase, coupling factor 6; mitochondrial ATP synthase, delta subunit; mitochondrial ATP synthase, gamma subunit 1; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthase, subunit 9, isoform 1; mitochondrial ATP synthase, subunit C (subunit 9), isoform 2; mitochondrial ATP synthase, subunit C, isoform 1; mitochondrial ATP synthase, subunit F6; mitochondrial ATP synthetase, beta subunit; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial ATPase; mitochondrial ATPase coupling factor 6; MLQ; My032 protein; Oligomycin sensitivity conferral protein; oligomycin sensitivity conferral protein oscp-like protein; oligomycin sensitivity conferring protein; OSCP; proliferation-inducing protein 36

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Gene Aliases: 0610008F14Rik; 0610009D10Rik; 1110019H14Rik; 1500000I11Rik; 1700094F02Rik; 2410043G19Rik; 2610008D24Rik; 4933437C06Rik; AA960090; AI876556; ATP5; ATP5A; ATP5A1; ATP5A2; ATP5AL2; ATP5B; ATP5C; ATP5C1; ATP5CL1; ATP5D; ATP5E; ATP5F1; ATP5F1A; ATP5F1B; ATP5F1C; ATP5F1D; ATP5F1E; ATP5G; ATP5G1; ATP5G2; ATP5G3; ATP5H; ATP5I; ATP5IF1; ATP5J; ATP5J2; ATP5JG; ATP5JL; ATP5K; ATP5L; ATP5MC1; ATP5MC2; ATP5MC3; ATP5ME; ATP5MF; ATP5MG; ATP5MJ; ATP5MPL; ATP5O; ATP5PB; ATP5PD; ATP5PF; ATP5PO; ATP6; ATP8; ATPASE6; ATPASE8; ATPE; ATPI; ATPIF1; ATPIP; ATPM; ATPMB; ATPO; ATPQ; ATPSB; AU020773; AV000645; BOS_13477; BOS_19537; BOS_22238; BOS_5573; C14orf2; C76477; C85518; CF6; COXPD22; D12Wsu28e; F6; hATP1; HEL-S-123m; HEL-S-271; HMC08D05; IP; Lfm-1; Lfm1; MC5DN3; MC5DN4; MOM2; MP68; MT-ATP6; MT-ATP8; MTATP6; MTATP8; My032; OMR; ORM; OSCP; P3; PIG47; PLPM; PRO1574; PSEC0033