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          • Primary Antibodies ›
          • ATP10A Antibodies

          Invitrogen

          ATP10A Polyclonal Antibody

          View all (4) ATP10A antibodies
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          Cite ATP10A Polyclonal Antibody

          • Antibody Testing Data (1)
          ATP10A Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          ATP10A Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          ATP10A Antibody (PA5-120400) in WB

          Western blot analysis of ATP10A in extracts of various cell lines. Samples were incubated with ATP10A Polyclonal antibody (Product # PA5-120400) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          ATP10A Antibody in Western Blot (WB)

          Product Details

          PA5-120400

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 440-690 of human ATP10A (NP_077816.1).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.62 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2913972

          Product Specific Information

          Positive test controls include: A-549, U-251MG, DU-145, OVCAR3, HT-29, A-431. The target is usually found in the following locations: Cell membrane, Endoplasmic reticulum membrane, Multi-pass membrane protein.

          Immunogen sequence: RRCTVSGVEY SHDANAQRLA RYQEADSEEE EVVPRGGSVS QRGSIGSHQS VRVVHRTQST KSHRRTGSRA EAKRASMLSK HTAFSSPMEK DITPDPKLLE KVSECDKSLA VARHQEHLLA HLSPELSDVF DFFIALTICN TVVVTSPDQP RTKVRVRFEL KSPVKTIEDF LRRFTPSCLT SGCSSIGSLA ANKSSHKLGS SFPSTPSSDG MLLRLEERLG QPTSAIASNG YSSQADNWAS ELAQEQESER E

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Aminophospholipid translocase VA; ATPase class V type 10A; ATPase type IV, phospholipid transporting (P-type); ATPase, class V, type 10A; ATPase, Class V, type 10C; P4-ATPase flippase complex alpha subunit ATP10A; Phospholipid-transporting ATPase VA

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          Gene Aliases: ATP10A; ATP10C; ATPVA; ATPVC; KIAA0566

          View more View less

          UniProt ID: (Human) O60312

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          Entrez Gene ID: (Human) 57194

          View more View less

          Function(s)
          magnesium ion binding phospholipid-translocating ATPase activity protein binding ATP binding
          Process(es)
          regulation of cell shape ion transmembrane transport phospholipid translocation
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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