Invitrogen

ATP1A1 Monoclonal Antibody (9-A5)

ATP1A1 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 6

ATP1A1 Antibody (MA3-924) in IHC

Immunohistochemistry was performed on human renal carcinoma tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sodiµm/Potassium ATPase mouse monoclonal antibody (Product # MA3-924) at a dilution of 1:10 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed... View More

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Product Details

MA3-924

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1/100

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

1/50

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Neutralization (Neu)

View 2 publications 2 publications

Inhibition Assays (IA)

Assay-dependent

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

9-A5

Immunogen

Purified rat kidney sodium/potassium ATPase.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2061129

Product Specific Information

MA3-924 detects the alpha 1 sodium/potassium ATPase in human, mouse, canine, rat and avian tissues.

MA3-924 has been successfully used in immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA and immunoprecipitation procedures. Immunohistochemical staining of sodium/potassium ATPase in rat liver with MA3-924 yields a pattern consistent with plasma membrane localization. Immunoprecipitation of sodium/potassium ATPase from canine kidney extracts can be used for affinity purification of the alpha subunit. MA3-924 can also be utilized to inhibit activity of the enzyme.

The MA3-924 antigen is purified rat kidney sodium/potassium ATPase. This antibody recognizes an epitope within an intracellular region at or near Asp-369 of the sodium/potassium ATPase alpha subunit.

Target Information

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Characterization of CFTR High Expresser cells in the intestine.

American journal of physiology. Gastrointestinal and liver physiology

Jakab RL,Collaco AM,Ameen NA

MA3-924 was used in immunohistochemistry to study the function of CFTR High Expresser cells in the intestine of rats

Sun Sep 15 00:00:00 UTC 2013

Lubiprostone targets prostanoid signaling and promotes ion transporter trafficking, mucus exocytosis, and contractility.

Digestive diseases and sciences

Jakab RL,Collaco AM,Ameen NA

Published figure using ATP1A1 monoclonal antibody (Product # MA3-924) in Immunofluorescence

Thu Nov 01 00:00:00 UTC 2012

Involvement of IL-10 in exhaustion of myeloid dendritic cells and rescue by CD40 stimulation.

Immunology

Kajino K,Nakamura I,Bamba H,Sawai T,Ogasawara K

MA3-924 was used in ELISA to study the role of interleukin 10 in the function of myeloid dendritic cells

Mon Jan 01 00:00:00 UTC 2007

The tetraspanin protein peripherin-2 forms a complex with melanoregulin, a putative membrane fusion regulator.

Biochemistry

Boesze-Battaglia K,Song H,Sokolov M,Lillo C,Pankoski-Walker L,Gretzula C,Gallagher B,Rachel RA,Jenkins NA,Copeland NG,Morris F,Jacob J,Yeagle P,Williams DS,Damek-Poprawa M

MA3-924 was used in immunocytochemistry to study the interaction between tetraspanin protein peripherin-2 and melanoregulin

Tue Feb 06 00:00:00 UTC 2007

The tetraspanin protein peripherin-2 forms a complex with melanoregulin, a putative membrane fusion regulator.

Biochemistry

Boesze-Battaglia K,Song H,Sokolov M,Lillo C,Pankoski-Walker L,Gretzula C,Gallagher B,Rachel RA,Jenkins NA,Copeland NG,Morris F,Jacob J,Yeagle P,Williams DS,Damek-Poprawa M

MA3-924 was used in immunocytochemistry to study the interaction between tetraspanin protein peripherin-2 and melanoregulin

Tue Feb 06 00:00:00 UTC 2007

A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.

The Journal of biological chemistry

Venkatesan S,Petrovic A,Locati M,Kim YO,Weissman D,Murphy PM

MA3-924 was used in immunohistochemistry to study the mechanism in CCR5 cell surface expression.

Fri Oct 26 00:00:00 UTC 2001

Hepatic Na(+)-K(+)-ATPase enzyme activity correlates with polarized beta-subunit expression.

The American journal of physiology

Simon FR,Leffert HL,Ellisman M,Iwahashi M,Deerinck T,Fortune J,Morales D,Dahl R,Sutherland E

MA3-924 was used in immunohistochemistry to investigate the relationship between hepatic sodium/potassium ATPase activity and the expression of polarized beta subunit

Sat Jul 01 00:00:00 UTC 1995

Identification of monoclonal antibody binding domains of Na+,K(+)-ATPase by immunoelectron microscopy.

FEBS letters

Ting-Beall HP,Beall HC,Hastings DF,Friedman ML,Ball WJ

MA3-924 was used in crystallagraphy to investigate the binding domains of sodium/potassium ATPase antibody.

Mon Jun 04 00:00:00 UTC 1990

Rat hepatic (Na+, K+)-ATPase: alpha-subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping.

Biochemistry

Hubert JJ,Schenk DB,Skelly H,Leffert HL

MA3-924 was used in neutralization experiments in rat liver

Tue Jul 15 00:00:00 UTC 1986

Use of a monoclonal antibody to quantify (Na+,K+)-ATPase activity and sites in normal and regenerating rat liver.

The Journal of biological chemistry

Schenk DB,Hubert JJ,Leffert HL

MA3-924 was used in blocking/activating experiment to investigate the activity and sites of sodium/potassium ATPase in normal and regenerating rat liver

Mon Dec 10 00:00:00 UTC 1984

Bioinformatics

Protein Aliases: (Na+ + K+)-ATPase; ATP1B; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; RP4-655J12.1; Sodium Potassium ATPase; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; Sodium/potassium-transporting ATPase subunit alpha-1

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Gene Aliases: ATP1A1; Atpa-1; BC010319; Nkaa1b

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UniProt ID: (Human) P05023, (Chicken) P09572, (Rat) P06685, (Mouse) Q8VDN2

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Entrez Gene ID: (Dog) 403992, (Human) 476, (Chicken) 396530, (Rat) 24211, (Mouse) 11928

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Function(s)

sodium:potassium-exchanging ATPase activity ATP binding metal ion binding protein binding phosphatase activity protein kinase binding protein domain specific binding ankyrin binding potassium ion binding sodium ion binding ADP binding phosphatidylinositol 3-kinase binding chaperone binding steroid hormone binding nucleotide binding hydrolase activity

Process(es)

regulation of sodium ion transport potassium ion transport sodium ion transport metabolic process establishment or maintenance of transmembrane electrochemical gradient ATP hydrolysis coupled proton transport regulation of the force of heart contraction cellular sodium ion homeostasis regulation of blood pressure potassium ion import energy coupled proton transmembrane transport, against electrochemical gradient dephosphorylation cellular potassium ion homeostasis negative regulation of glucocorticoid biosynthetic process sodium ion transmembrane transport sodium ion export from cell response to drug negative regulation of heart contraction positive regulation of heart contraction positive regulation of striated muscle contraction relaxation of cardiac muscle cardiac muscle contraction membrane hyperpolarization cellular response to mechanical stimulus cellular response to steroid hormone stimulus potassium ion transmembrane transport regulation of cardiac muscle cell contraction membrane repolarization response to glycoside ion transmembrane transport membrane repolarization during cardiac muscle cell action potential cell communication by electrical coupling involved in cardiac conduction regulation of cardiac conduction potassium ion import across plasma membrane transport ion transport ATP biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATP1A1 Monoclonal Antibody (9-A5)

ATP1A1 Antibody (MA3-924) in IHC

Product Details

MA3-924

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Inhibition Assays (IA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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