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Invitrogen
ATP1A3 Polyclonal Antibody
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ATP1A3 Antibody (PA5-90893) in WB
Western blot analysis of ATP1A3 in extracts of various cell lines. Samples were incubated with ATP1A3 Polyclonal antibody (Product # PA5-90893) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-90893
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human ATP1A3 (NP_6895091)
Conjugate
Form
Liquid
Concentration
0.76 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806277
Product Specific Information
Immunogen sequence: MGDKKDDKDS PKKNKGKERR DLDDLKKEVA MTEHKMSVEE VCRKYNTDCV QGLTHSKAQE; Positive Samples: Mouse liver, Rat liver; Cellular Location: Cell membrane, Multi-pass membrane protein
Target Information
The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase, Na+K+ transporting, alpha 3 subunit; EC 3.6.3.9; Na(+)/K(+) ATPase alpha(III) subunit; Na(+)/K(+) ATPase alpha-3 subunit; Na+/K+ -ATPase alpha 3 subunit; Sodium Potassium ATPase; Sodium pump 3; Sodium pump subunit alpha-3; Sodium/potassium-transporting ATPase subunit alpha-3
Gene Aliases: Atp1a3; Atpa-2; Atpa1a3
UniProt ID: (Rat) P06687, (Mouse) Q6PIC6
Entrez Gene ID: (Rat) 24213, (Mouse) 232975
sodium:potassium-exchanging ATPase activity
protein binding
ATP binding
cation-transporting ATPase activity
D1 dopamine receptor binding
metal ion binding
chaperone binding
nucleotide binding
hydrolase activity
heparan sulfate proteoglycan binding
sodium:potassium-exchanging ATPase activity involved in regulation of cardiac muscle cell membrane potential
potassium ion transport
sodium ion transport
cellular sodium ion homeostasis
memory
metabolic process
adult locomotory behavior
visual learning
potassium ion import
ATP hydrolysis coupled proton transport
cellular potassium ion homeostasis
ionotropic glutamate receptor signaling pathway
sodium ion export from cell
response to drug
transport
ion transport
sodium ion transmembrane transport
cardiac muscle contraction
potassium ion transmembrane transport
regulation of cardiac muscle cell membrane potential
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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