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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • ATP1B1 Antibodies

          Invitrogen

          ATP1B1 Monoclonal Antibody (M17-P5-F11)

          Advanced Verification
          26 Published Figures
          57 References
          View all (10) ATP1B1 antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite ATP1B1 Monoclonal Antibody (M17-P5-F11)

          • Antibody Testing Data (7)
          • Published Figures (26)
          • Advanced Verification (1)
          ATP1B1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          ATP1B1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 34

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          ATP1B1 Antibody (MA3-930) in ICC/IF

          Immunofluorescent analysis of Sodiµm/Potassium ATPase beta using Anti-Sodiµm/Potassium ATPase beta Monoclonal Antibody (M17-P5-F11) (Product # MA3-930) shows staining in MCF-7 Cells. Sodiµm/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodiµm/Potassium ATPase beta (Product # MA3-930) at a dilution of 1:200 over night at... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          ATP1B1 Antibody in Immunocytochemistry (ICC/IF)
          ATP1B1 Antibody in Immunocytochemistry (ICC/IF)
          ATP1B1 Antibody in Immunocytochemistry (ICC/IF)
          ATP1B1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP1B1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP1B1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Immunocytochemistry (ICC/IF)
          ATP1B1 Antibody in Immunohistochemistry (IHC)
          ATP1B1 Antibody in Immunohistochemistry (IHC)
          ATP1B1 Antibody in Immunohistochemistry (IHC)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody in Western Blot (WB)
          ATP1B1 Antibody

          Product Details

          MA3-930

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:10,000
          View 47 publications 47 publications

          Immunohistochemistry (IHC)

          -
          View 3 publications 3 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          View 4 publications 4 publications

          Immunoprecipitation (IP)

          Assay-dependent
          -

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Dog, Human, Mouse, Sheep, Pig

          Published species

          Dog, Fruit fly, Human, Invertebrate, Mouse, Sheep

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          M17-P5-F11

          Immunogen

          Purified lamb kidney beta sodium/potassium ATPase.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2060990

          Product Specific Information

          MA3-930 detects sodium/potassium ATPase beta 1 from mouse, human, sheep, canine, and pig tissues. This antibody does not recognize the beta 1 or beta 2 subunit in rat.

          MA3-930 has been successfully used in Western blot and immunohistochemistry and immunocytochemistry procedures. By Western blot, this antibody detects an ~42 kDa protein in liver samples and ~55 kDa in kidney samples representing beta sodium/potassium ATPase in sheep liver extract. Immunohistochemical staining of the sodium/potassium ATPase with MA3-930 yields a pattern consistent with plasma membrane localization.

          The MA3-930 antigen is purified lamb kidney sodium/potassium ATPase beta. This antibody recognizes and epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.

          Target Information

          The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: adenosinetriphosphatase; ATPase beta subunit; ATPase, Na+/K+ transporting, beta 1 polypeptide; Beta 1-subunit of Na(+),K(+)-ATPase; MGC1798; Na+, K+-ATPase beta-subunit; Na+/K+ ATPase Beta; Na, K-ATPase beta-1 polypeptide; OTTHUMP00000032537; OTTHUMP00000032538; Sodium Potassium ATPase; sodium pump subunit beta-1; sodium-potassium ATPase subunit beta 1 (non-catalytic); sodium/potassium ATPase beta subunit; sodium/potassium-dependent ATPase beta-1 subunit; Sodium/potassium-dependent ATPase subunit beta-1; sodium/potassium-transporting ATPase beta-1 chain; Sodium/potassium-transporting ATPase subunit beta-1; unnamed protein product

          View more View less

          Gene Aliases: ATP1B; ATP1B1; Atp4b; Atpb; Atpb-1; NKbeta1

          View more View less

          UniProt ID: (Sheep) P05028, (Pig) P05027, (Human) P05026, (Mouse) P14094

          View more View less

          Entrez Gene ID: (Sheep) 443384, (Dog) 403966, (Pig) 396898, (Human) 481, (Mouse) 11931

          View more View less

          Function(s)
          ATPase activator activity sodium:potassium-exchanging ATPase activity protein binding ATP binding protein C-terminus binding drug binding ATPase activity MHC class II protein complex binding potassium ion binding sodium ion binding ATPase binding
          Process(es)
          potassium ion transport sodium ion transport cell adhesion response to hypoxia positive regulation of defense response to virus by host cellular calcium ion homeostasis cellular sodium ion homeostasis potassium ion import establishment or maintenance of transmembrane electrochemical gradient regulation of gene expression regulation of cardiac muscle contraction by calcium ion signaling cellular potassium ion homeostasis positive regulation of ATPase activity ion transmembrane transport sodium ion export from cell protein transport into plasma membrane raft ATP metabolic process protein stabilization leukocyte migration relaxation of cardiac muscle cardiac muscle contraction protein localization to plasma membrane membrane repolarization membrane repolarization during cardiac muscle cell action potential cell communication by electrical coupling involved in cardiac conduction ATP hydrolysis coupled transmembrane transport mitophagy in response to mitochondrial depolarization xenophagy positive regulation of potassium ion transmembrane transporter activity positive regulation of sodium ion export from cell positive regulation of calcium:sodium antiporter activity positive regulation of potassium ion import regulation of cardiac conduction potassium ion import across plasma membrane blastocyst development transport ion transport metal ion transport regulation of calcium ion transmembrane transport
          It has to be done as per old AB suggested Products section.
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