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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • ATP4B Antibodies

          Invitrogen

          ATP4B Monoclonal Antibody (2G11)

          17 Published Figures
          59 References
          View all (11) ATP4B antibodies

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          Datasheet
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          Datasheet
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          Cite ATP4B Monoclonal Antibody (2G11)

          • Antibody Testing Data (5)
          • Published Figures (17)
          ATP4B Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          ATP4B Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 22

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          ATP4B Antibody (MA3-923) in ICC/IF

          Immunofluorescent analysis of Hydrogen/Potassium ATPase beta using Anti-Hydrogen/Potassium ATPase beta Monoclonal Antibody (2G11) (Product # MA3-923) shows staining in A2058 Cells. Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta (Product # MA3-923) at a dilution of 1:100 over night ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          ATP4B Antibody in Immunocytochemistry (ICC/IF)
          ATP4B Antibody in Immunocytochemistry (ICC/IF)
          ATP4B Antibody in Immunocytochemistry (ICC/IF)
          ATP4B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP4B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP4B Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          ATP4B Antibody in Immunohistochemistry (IHC)
          ATP4B Antibody in Immunohistochemistry (IHC)
          ATP4B Antibody in Immunohistochemistry (IHC)
          ATP4B Antibody in Immunohistochemistry (IHC)
          ATP4B Antibody in Immunohistochemistry (IHC)
          ATP4B Antibody in Immunohistochemistry (IHC)
          ATP4B Antibody in Immunohistochemistry (IHC)
          ATP4B Antibody in Immunohistochemistry (IHC)
          ATP4B Antibody in Immunohistochemistry (IHC)
          ATP4B Antibody in Immunohistochemistry (IHC)
          ATP4B Antibody in Western Blot (WB)
          ATP4B Antibody in Western Blot (WB)
          ATP4B Antibody in Flow Cytometry (Flow)
          ATP4B Antibody in Flow Cytometry (Flow)
          ATP4B Antibody in Flow Cytometry (Flow)
          ATP4B Antibody in Flow Cytometry (Flow)

          Product Details

          MA3-923

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:4,000
          View 4 publications 4 publications

          Immunohistochemistry (IHC)

          -
          View 37 publications 37 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:2,000
          View 2 publications 2 publications

          Immunohistochemistry (Frozen) (IHC (F))

          1:2,000
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          View 11 publications 11 publications

          Flow Cytometry (Flow)

          1/50
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          Assay-dependent
          View 1 publication 1 publication

          ChIP assay (ChIP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Bovine, Dog, Human, Mustelid, Mouse, Pig, Rabbit, Rat

          Published species

          Bovine, Guinea pig, Human, Mouse, Rabbit, Rat, Rodent

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          2G11

          Immunogen

          Purified 34 kDa core peptide from deglycosylated hog gastric microsomes.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          PBS, ascites

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2227726

          Product Specific Information

          MA3-923 detects the beta-subunit of hydrogen/potassium ATPase from bovine, human, canine, porcine, rabbit, mouse, ferret, and rat tissues.

          MA3-923 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation procedures (ChIP). By Western blot, this antibody detects various forms of the beta-subunit, including a 60 to 80 kDa glycosylated protein, a 52 kDa beta-subunit precursor, and the 34 kDa core peptide from rabbit stomach extracts. Immunohistochemical staining of hydrogen/potassium ATPase in rabbit gastric glands with MA3-923 results in specific staining of parietal cells.

          MA3-923 has been shown to inhibit the enzymatic activity of the hydrogen/potassium ATPase and to alter the affinity of the cytoplasmic potassium binding site.

          The MA3-923 antigen is purified 34 kDa core peptide from deglycosylated hog gastric microsomes. This antibody recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit.

          Target Information

          The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha- subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ATPase H+K+-transporting beta / (gastric HK-ATPase beta subunit) defined by SSR; ATPase, H+,K+-transporting, beta / (gastric H,K-ATPase beta subunit), defined by SSR; ATPase, H+/K+ exchanging, beta polypeptide; ATPase, H+/K+ transporting, beta polypeptide; ATPase, H+/K+ transporting, beta polypeptide, gastric specific; Gastric H(+)/K(+) ATPase subunit beta; gastric H+/K+ ATPase beta subunit; gastric H+/K+-ATPase beta subunit; gastric hydrogen-potassium ATPase, beta; gp60-90; H+,K+-ATPase; H+/K+ ATPase beta; H+/K+-ATPase beta; H,K-ATPase-Beta; H.K-ATPase, beta subunit; potassium-transporting ATPase beta chain; Potassium-transporting ATPase subunit beta; Proton pump beta chain

          View more View less

          Gene Aliases: ATP4B; ATP6B; AV080843; BOS_12738; S76401S1

          View more View less

          UniProt ID: (Pig) P18434, (Rabbit) P18597, (Human) P51164, (Rat) P18598, (Mouse) P50992

          View more View less

          Entrez Gene ID: (Pig) 397131, (Rabbit) 100009125, (Human) 496, (Bovine) 614308, (Dog) 404020, (Rat) 24217, (Mouse) 11945

          View more View less

          Function(s)
          protein binding hydrogen:potassium-exchanging ATPase activity protein heterodimerization activity
          Process(es)
          gastric acid secretion potassium ion transport sodium ion transport cell adhesion response to organonitrogen compound response to lipopolysaccharide hydrogen ion transmembrane transport ion transmembrane transport proton transport transport ion transport regulation of proton transport protein glycosylation in endoplasmic reticulum pH reduction potassium ion transmembrane transport
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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