Proteintech

ATP5A1 Monoclonal Antibody (1B10H3)

ATP5A1 Antibody in Immunocytochemistry (ICC/IF)

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ATP5A1 Antibody (66037-1-IG) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATP5A1 antibody (Product # 66037-1-Ig) at dilution of 1:300 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: CL555-phalloidin staining of F-actin.

ATP5A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATP5A1 Antibody in Flow Cytometry (Flow)

ATP5A1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

66037-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1B10H3

Immunogen

ATP5A1 Fusion Protein Ag8119 (202-553 aa encoded by BC064562)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.95 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.

Immunogen sequence: IGRGQRELI IGDRQTGKTS IAIDTIINQK RFNDGSDEKK KLYCIYVAIG QKRSTVAQLV KRLTDADAMK YTIVVSATAS DAAPLQYLAP YSGCSMGEYF RDNGKHALII YDDLSKQAVA YRQMSLLLRR PPGREAYPGD VFYLHSRLLE RAAKMNDAFG GGSLTALPVI ETQAGDVSAY IPTNVISITD GQIFLETELF YKGIRPAINV GLSVSRVGSA AQTRAMKQVA GTMKLELAQY REVAAFAQFG SDLDAATQQL LSRGVRLTEL LKQGQYSPMA IEEQVAVIYA GVRGYLDKLE PSKITKFENA FLSHVVSQHQ ALLGTIRADG KISEQSDAKL KEIVTNFLAG FEA (202-553 aa encoded by BC064562)

Target Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP synthase alpha chain, mitochondrial; ATP synthase F1 subunit alpha; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; epididymis secretory sperm binding protein Li 123m; iso; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial H+-ATP synthase alpha subunit; modifier of Min 2

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Gene Aliases: AI035633; AL022851; AL023067; ATP5A; ATP5A1; ATP5AL2; ATP5F1A; ATPM; COXPD22; D18Ertd206e; hATP1; HEL-S-123m; MC5DN4; MOM2; OMR; ORM

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UniProt ID: (Human) P25705, (Rat) P15999, (Mouse) Q03265

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Entrez Gene ID: (Human) 498, (Rat) 65262, (Mouse) 11946

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Function(s)

protein binding ATP binding ATPase activity transmembrane transporter activity MHC class I protein binding poly(A) RNA binding proton-transporting ATP synthase activity, rotational mechanism proton-transporting ATPase activity, rotational mechanism ADP binding nucleotide binding hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances

Process(es)

negative regulation of endothelial cell proliferation lipid metabolic process ATP synthesis coupled proton transport ATP hydrolysis coupled proton transport ATP metabolic process hydrogen ion transmembrane transport ATP biosynthetic process transport ion transport embryo development proton transport mitochondrial ATP synthesis coupled proton transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATP5A1 Monoclonal Antibody (1B10H3)

ATP5A1 Antibody (66037-1-IG) in ICC/IF

Product Details

66037-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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