Proteintech

ATP5F1 Polyclonal Antibody

ATP5F1 Antibody in Immunocytochemistry (ICC/IF)

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ATP5F1 Antibody (15999-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using ATP5F1 antibody 15999-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

ATP5F1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

15999-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ATP5F1 Fusion Protein Ag8571 (1-256 aa encoded by BC005366)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MLSRVVLSA AATAAPSLKN AAFLGPGVLQ ATRTFHTGQP HLVPVPPLPE YGGKVRYGLI PEEFFQFLYP KTGVTGPYVL GTGLILYALS KEIYVISAET FTALSVLGVM VYGIKKYGPF VADFADKLNE QKLAQLEEAK QASIQHIQNA IDTEKSQQAL VQKRHYLFDV QRNNIAMALE VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST QQEKETIAKC IADLKLLAKK AQAQPVM (1-256 aa encoded by BC005366)

Target Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A high-fat diet increases hepatic mitochondrial turnover through restricted acetylation in a NAFLD mouse model.

American journal of physiology. Endocrinology and metabolism

Aghayev M,Arias-Alvarado A,Ilchenko S,Lepp J,Scott I,Chen YR,Zhang GF,Tsai TH,Kasumov T

Sat Jul 01 00:00:00 UTC 2023

Human TRUB1 is a highly conserved pseudouridine synthase responsible for the formation of Ψ55 in mitochondrial tRNAAsn, tRNAGln, tRNAGlu and tRNAPro.

Nucleic acids research

Jia Z,Meng F,Chen H,Zhu G,Li X,He Y,Zhang L,He X,Zhan H,Chen M,Ji Y,Wang M,Guan MX

Fri Sep 09 00:00:00 UTC 2022

Bromodomain-containing protein 4 (BRD4) as an epigenetic regulator of fatty acid metabolism genes and ferroptosis.

Cell death & disease

Yang M,Liu K,Chen P,Zhu H,Wang J,Huang J

Sat Oct 29 00:00:00 UTC 2022

Mitochondrial and glucose metabolic dysfunctions in granulosa cells induce impaired oocytes of polycystic ovary syndrome through Sirtuin 3.

Free radical biology & medicine

Zhang Q,Ren J,Wang F,Pan M,Cui L,Li M,Qu F

Fri Jul 01 00:00:00 UTC 2022

ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer.

Journal of experimental & clinical cancer research : CR

Dong S,Liang S,Cheng Z,Zhang X,Luo L,Li L,Zhang W,Li S,Xu Q,Zhong M,Zhu J,Zhang G,Hu S

Sat Jan 08 00:00:00 UTC 2022

GABA regulates IL-1β production in macrophages.

Cell reports

Fu J,Han Z,Wu Z,Xia Y,Yang G,Yin Y,Ren W

Tue Dec 06 00:00:00 UTC 2022

Acetylation of Atp5f1c Mediates Cardiomyocyte Senescence via Metabolic Dysfunction in Radiation-Induced Heart Damage.

Oxidative medicine and cellular longevity

Zeng Z,Xu P,He Y,Yi Y,Liu Z,Cai J,Huang L,Liu A

Wed Sep 28 00:00:00 UTC 2022

Acetylation of Atp5f1c Mediates Cardiomyocyte Senescence via Metabolic Dysfunction in Radiation-Induced Heart Damage.

Oxidative medicine and cellular longevity

Zeng Z,Xu P,He Y,Yi Y,Liu Z,Cai J,Huang L,Liu A

Wed Sep 28 00:00:00 UTC 2022

Acetylation of Atp5f1c Mediates Cardiomyocyte Senescence via Metabolic Dysfunction in Radiation-Induced Heart Damage.

Oxidative medicine and cellular longevity

Zeng Z,Xu P,He Y,Yi Y,Liu Z,Cai J,Huang L,Liu A

Wed Sep 28 00:00:00 UTC 2022

Acetylation of Atp5f1c Mediates Cardiomyocyte Senescence via Metabolic Dysfunction in Radiation-Induced Heart Damage.

Oxidative medicine and cellular longevity

Zeng Z,Xu P,He Y,Yi Y,Liu Z,Cai J,Huang L,Liu A

Wed Sep 28 00:00:00 UTC 2022

Transcriptomics of single dose and repeated carbon black and ozone inhalation co-exposure highlight progressive pulmonary mitochondrial dysfunction.

Particle and fibre toxicology

Hathaway QA,Majumder N,Goldsmith WT,Kunovac A,Pinti MV,Harkema JR,Castranova V,Hollander JM,Hussain S

Wed Dec 15 00:00:00 UTC 2021

Transcriptomics of single dose and repeated carbon black and ozone inhalation co-exposure highlight progressive pulmonary mitochondrial dysfunction.

Particle and fibre toxicology

Hathaway QA,Majumder N,Goldsmith WT,Kunovac A,Pinti MV,Harkema JR,Castranova V,Hollander JM,Hussain S

Wed Dec 15 00:00:00 UTC 2021

An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.

The Journal of biological chemistry

Jin X,Zhang Z,Nie Z,Wang C,Meng F,Yi Q,Chen M,Sun J,Zou J,Jiang P,Guan MX

Mon Aug 02 00:00:00 UTC 2021

Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9.

Virology journal

Yang Y,Zhang Y,Yang C,Fang F,Wang Y,Chang H,Chen Z,Chen P

Thu Feb 18 00:00:00 UTC 2021

Spermine Protects Cardiomyocytes from High Glucose-Induced Energy Disturbance by Targeting the CaSR-gp78-Ubiquitin Proteasome System.

Cardiovascular drugs and therapy

Wang Y,Wang Y,Li F,Zhang X,Li H,Yang G,Xu C,Wei C

Mon Feb 01 00:00:00 UTC 2021

COA6 Facilitates Cytochrome c Oxidase Biogenesis as Thiol-reductase for Copper Metallochaperones in Mitochondria.

Journal of molecular biology

Pacheu-Grau D,Wasilewski M,Oeljeklaus S,Gibhardt CS,Aich A,Chudenkova M,Dennerlein S,Deckers M,Bogeski I,Warscheid B,Chacinska A,Rehling P

Fri Mar 27 00:00:00 UTC 2020

Heat Stress Impairs the Physiological Responses and Regulates Genes Coding for Extracellular Exosomal Proteins in Rat.

Genes

Dou J,Khan A,Khan MZ,Mi S,Wang Y,Yu Y,Wang Y

Fri Mar 13 00:00:00 UTC 2020

A Mutation in Hnrnph1 That Decreases Methamphetamine-Induced Reinforcement, Reward, and Dopamine Release and Increases Synaptosomal hnRNP H and Mitochondrial Proteins.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ruan QT,Yazdani N,Blum BC,Beierle JA,Lin W,Coelho MA,Fultz EK,Healy AF,Shahin JR,Kandola AK,Luttik KP,Zheng K,Smith NJ,Cheung J,Mortazavi F,Apicco DJ,Ragu Varman D,Ramamoorthy S,Ash PEA,Rosene DL,Emili A,Wolozin B,Szumlinski KK,Bryant CD

Thu Jan 02 00:00:00 UTC 2020

Urea transport B gene induces melanoma B16 cell death via activation of p53 and mitochondrial apoptosis.

Cancer science

Liu L,Sun Y,Zhao Y,Wang Q,Guo H,Guo R,Liu Y,Fu S,Zhang L,Li Y,Meng Y

Sat Dec 01 00:00:00 UTC 2018

Urea transport B gene induces melanoma B16 cell death via activation of p53 and mitochondrial apoptosis.

Cancer science

Liu L,Sun Y,Zhao Y,Wang Q,Guo H,Guo R,Liu Y,Fu S,Zhang L,Li Y,Meng Y

Sat Dec 01 00:00:00 UTC 2018

Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.

Cell death & disease

Wang Y,Gao P,Wei C,Li H,Zhang L,Zhao Y,Wu B,Tian Y,Zhang W,Wu L,Wang R,Xu C

Thu May 18 00:00:00 UTC 2017

Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.

Cell death & disease

Wang Y,Gao P,Wei C,Li H,Zhang L,Zhao Y,Wu B,Tian Y,Zhang W,Wu L,Wang R,Xu C

Thu May 18 00:00:00 UTC 2017

F1FO ATP Synthase Is Expressed at the Surface of Embryonic Rat Heart-Derived H9c2 Cells and Is Affected by Cardiac-Like Differentiation.

Journal of cellular biochemistry

Comelli M,Domenis R,Buso A,Mavelli I

Mon Feb 01 00:00:00 UTC 2016

F1FO ATP Synthase Is Expressed at the Surface of Embryonic Rat Heart-Derived H9c2 Cells and Is Affected by Cardiac-Like Differentiation.

Journal of cellular biochemistry

Comelli M,Domenis R,Buso A,Mavelli I

Mon Feb 01 00:00:00 UTC 2016

Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex.

FEBS letters

Fujikawa M,Sugawara K,Tanabe T,Yoshida M

Mon Sep 14 00:00:00 UTC 2015

Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex.

FEBS letters

Fujikawa M,Sugawara K,Tanabe T,Yoshida M

Mon Sep 14 00:00:00 UTC 2015

Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation.

Proceedings of the National Academy of Sciences of the United States of America

Kioka H,Kato H,Fujikawa M,Tsukamoto O,Suzuki T,Imamura H,Nakano A,Higo S,Yamazaki S,Matsuzaki T,Takafuji K,Asanuma H,Asakura M,Minamino T,Shintani Y,Yoshida M,Noji H,Kitakaze M,Komuro I,Asano Y,Takashima S

Tue Jan 07 00:00:00 UTC 2014

Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation.

Proceedings of the National Academy of Sciences of the United States of America

Tue Jan 07 00:00:00 UTC 2014

Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells.

Journal of agricultural and food chemistry

Liu C,Lin JJ,Yang ZY,Tsai CC,Hsu JL,Wu YJ

Wed Dec 03 00:00:00 UTC 2014

Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells.

Journal of agricultural and food chemistry

Liu C,Lin JJ,Yang ZY,Tsai CC,Hsu JL,Wu YJ

Wed Dec 03 00:00:00 UTC 2014

Ech1 is a potent suppressor of lymphatic metastasis in hepatocarcinoma.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zhang J,Sun M,Li R,Liu S,Mao J,Huang Y,Wang B,Hou L,Ibrahim MM,Tang J

Sun Sep 01 00:00:00 UTC 2013

Ech1 is a potent suppressor of lymphatic metastasis in hepatocarcinoma.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zhang J,Sun M,Li R,Liu S,Mao J,Huang Y,Wang B,Hou L,Ibrahim MM,Tang J

Sun Sep 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: ATP synthase B chain, mitochondrial; ATP synthase F(0) complex subunit B1, mitochondrial; ATP synthase peripheral stalk-membrane subunit b; ATP synthase proton-transporting mitochondrial F(0) complex subunit B1; ATP synthase subunit b; ATP synthase subunit b, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1; ATPase subunit b; cell proliferation-inducing protein 47; H+-ATP synthase subunit b

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Gene Aliases: Atp5f; ATP5F1; ATP5PB; C76477; PIG47

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UniProt ID: (Human) P24539, (Mouse) Q9CQQ7, (Rat) P19511

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Entrez Gene ID: (Human) 515, (Mouse) 11950, (Rat) 171375

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Function(s)

protein binding ATPase activity transmembrane transporter activity proton-transporting ATP synthase activity, rotational mechanism

Process(es)

ATP synthesis coupled proton transport ATP metabolic process ATP biosynthetic process substantia nigra development mitochondrial ATP synthesis coupled proton transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATP5F1 Polyclonal Antibody

ATP5F1 Antibody (15999-1-AP) in ICC/IF

Product Details

15999-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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