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Invitrogen
ATP5G1/2/3 Polyclonal Antibody
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ATP5G1/2/3 Antibody (PA5-114397) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of ATP5G1/ATP5G2/ATP5G3 in A549 cells using ATP5G1/2/3 Polyclonal Antibody (Product # PA5-114397). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibod... View More
Product Details
PA5-114397
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human ATP5F1/ATP5MC1 recombinant protein (Position: D62-L113).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2884853
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HEPG2 whole cell, human A549 whole cell, human PC-3 whole cell, human HL-60 whole cell, human HEK293 whole cell, human U937 whole cell, human CACO-2 whole cell, rat intestine tissue, human Ovarian cancer tissue, human tonsil tissue, human glioma tissue, human lung cancer tissue, human placenta tissue, mouse intestine tissue. IHC: human intestinal tissue. Flow: HL-60 cell, PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The ATP5MC1 gene is one of three human paralogs that encode membrane subunit c of the mitochondrial ATP synthase. It is mapped to 17q21.32. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP synthase c subunit; ATP synthase F(0) complex subunit C1, mitochondrial; ATP synthase F(0) complex subunit C2, mitochondrial; ATP synthase F(0) complex subunit C3, mitochondrial; ATP synthase lipid-binding protein; ATP synthase lipid-binding protein, mitochondrial; ATP synthase membrane subunit c locus 1; ATP synthase membrane subunit c locus 2; ATP synthase membrane subunit c locus 3; ATP synthase proteolipid P1; ATP synthase proteolipid P2; ATP synthase proteolipid P3; ATP synthase proton-transporting mitochondrial F(0) complex subunit C1; ATP synthase proton-transporting mitochondrial F(0) complex subunit C2; ATP synthase proton-transporting mitochondrial F(0) complex subunit C3; ATP synthase subunit 9; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 2; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 3; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9); ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C2 (subunit 9); ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C2 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9); ATP synthase, mitochondrial, C subunit-3; ATPase protein 9; ATPase subunit 9; ATPase subunit C; mitochondrial ATP synthase, subunit 9, isoform 1; mitochondrial ATP synthase, subunit C (subunit 9), isoform 2; mitochondrial ATP synthase, subunit C, isoform 1
Gene Aliases: 1810041M08Rik; 6030447M23; ATP5A; ATP5G; ATP5G1; ATP5G2; ATP5G3; ATP5MC1; ATP5MC2; ATP5MC3; P3; PSEC0033
UniProt ID: (Human) P05496, (Human) Q06055, (Human) P48201, (Mouse) Q9CR84, (Rat) Q06646, (Mouse) P56383, (Mouse) P56384
Entrez Gene ID: (Human) 516, (Human) 517, (Human) 518, (Mouse) 11951, (Rat) 171082, (Mouse) 67942, (Mouse) 228033
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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