Invitrogen

ATP5H Monoclonal Antibody (7F9BG1)

ATP5H Antibody in Immunocytochemistry (ICC/IF)

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ATP5H Antibody (459000) in ICC/IF

Immunofluorescence analysis of ATP5H was performed using Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ATP5H Mouse Monoclonal Antibody (Product # 459000) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 for 45 min... View More

Product Image Gallery

ATP5H Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of ATP5H was observed in Mouse Heart (Lane 5) that expresses ATP5H protein at very high levels, in comparison to, Mouse Thymus (Lane 7) that expresses ATP5H protein at low levels using Anti-ATP5H Mouse Monoclonal Antibody (Product # 459000) in Western Blot. {RE}

Product Details

459000

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1-5 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/mL

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

7F9BG1

Immunogen

Bovine Complex V

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10374486

Product Specific Information

For mouse and rat samples, this antibody will only recognize ATP5H in purified mitochondrial samples. Mouse and rat cell lysates and tissue homogenates are not recommended with this antibody.

For positive control, use Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells.

Target Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ATM-mediated mitochondrial damage response triggered by nuclear DNA damage in normal human lung fibroblasts.

Cell cycle (Georgetown, Tex.)

Shimura T,Sasatani M,Kawai H,Kamiya K,Kobayashi J,Komatsu K,Kunugita N

459000 was used in Western Blotting to study the association between nuclear DNA damage and mitochondrial damage following ionizing radiation in normal human lung fibroblasts.

Tue Jul 02 00:00:00 UTC 2019

Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease.

Nature medicine

Lood C,Blanco LP,Purmalek MM,Carmona-Rivera C,De Ravin SS,Smith CK,Malech HL,Ledbetter JA,Elkon KB,Kaplan MJ

459000 was used in immunocytochemistry to elucidate enrichment of oxidized mitochondrial DNA in neutrophil extracellular traps contributes to lupus-like disease

Mon Feb 01 00:00:00 UTC 2016

Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex.

FEBS letters

Fujikawa M,Sugawara K,Tanabe T,Yoshida M

459000 was used in western blot to study the assembly process of human mitochondrial ATP synthase

Mon Sep 14 00:00:00 UTC 2015

Population of ATP synthase molecules in mitochondria is limited by available 6.8-kDa proteolipid protein (MLQ).

Genes to cells : devoted to molecular & cellular mechanisms

Fujikawa M,Ohsakaya S,Sugawara K,Yoshida M

459000 was used in immunocytochemistry to investigate MLQ as a regulator of mitochondrial ATP synthesis.

Sat Feb 01 00:00:00 UTC 2014

Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice.

Molecular neurodegeneration

Lasagna-Reeves CA,Castillo-Carranza DL,Sengupta U,Clos AL,Jackson GR,Kayed R

Published figure using ATP5A1 monoclonal antibody (Product # 459000) in Western Blot

Mon Jun 06 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: ATP synthase D chain, mitochondrial; ATP synthase peripheral stalk subunit d; ATP synthase subunit d, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d; ATP synthase, H+ transporting, mitochondrial F1F0, subunit d; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d; ATPase subunit d; My032 protein

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Gene Aliases: 0610009D10Rik; ATP5H; Atp5jd; ATP5PD; ATPQ; BOS_19537; My032

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UniProt ID: (Bovine) P13620, (Human) O75947, (Mouse) Q9DCX2, (Rat) P31399

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Entrez Gene ID: (Bovine) 282710, (Human) 10476, (Mouse) 71679, (Rat) 641434

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Function(s)

hydrogen ion transmembrane transporter activity ATPase activity transmembrane transporter activity proton-transporting ATP synthase activity, rotational mechanism

Process(es)

transport ion transport ATP synthesis coupled proton transport proton transport ATP metabolic process ATP biosynthetic process mitochondrial ATP synthesis coupled proton transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATP5H Monoclonal Antibody (7F9BG1)

ATP5H Antibody (459000) in ICC/IF

Product Details

459000

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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