Invitrogen

ATP7A Monoclonal Antibody (L60/4), PE

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-45521

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

L60/4

Immunogen

Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1
View immunogen

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931975

Product Specific Information

1 µg/mL of MA5-45521 was sufficient for detection of Copper-transporting ATPase1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.|Detects approximately 180kDa in rat brain membrane preparations.

This antibody was formerly sold as clone S60-4.

Target Information

ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP 7A; ATPase, Cu++ transporting, alpha polypeptide; Copper pump 1; Copper-transporting ATPase 1; Cu++-transporting P-type ATPase; FLJ17790; MC 1; Menke; Menkes disease-associated protein; Menkes disease-associated protein homolog; Menkes protein; Menkes syndrome; OHS; OTTHUMP00000062077

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Gene Aliases: ATP7A; DSMAX; MC1; MK; MNK; SMAX3

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UniProt ID: (Human) Q04656, (Mouse) Q64430, (Rat) P70705

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Entrez Gene ID: (Human) 538, (Mouse) 11977, (Rat) 24941

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Function(s)
copper-exporting ATPase activity copper ion transmembrane transporter activity copper ion binding protein binding ATP binding superoxide dismutase copper chaperone activity copper-dependent protein binding nucleotide binding hydrolase activity cation-transporting ATPase activity metal ion binding molecular_function
Process(es)
blood vessel development in utero embryonic development blood vessel remodeling regulation of oxidative phosphorylation tryptophan metabolic process catecholamine metabolic process copper ion transport cellular copper ion homeostasis mitochondrion organization lactation locomotory behavior response to iron(III) ion response to zinc ion detoxification of copper ion copper ion import plasma membrane copper ion transport peptidyl-lysine modification removal of superoxide radicals cerebellar Purkinje cell differentiation pyramidal neuron development central nervous system neuron development extracellular matrix organization collagen fibril organization hair follicle morphogenesis ion transmembrane transport T-helper cell differentiation epinephrine metabolic process norepinephrine metabolic process dopamine metabolic process serotonin metabolic process positive regulation of catalytic activity negative regulation of catalytic activity pigmentation skin development elastic fiber assembly lung alveolus development neuron projection morphogenesis cartilage development positive regulation of oxidoreductase activity elastin biosynthetic process copper ion export release of cytochrome c from mitochondria tyrosine metabolic process transport ion transport cation transport regulation of gene expression metal ion transport hindlimb morphogenesis norepinephrine biosynthetic process negative regulation of neuron apoptotic process ATP metabolic process response to copper ion dendrite morphogenesis
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