Invitrogen

ATPIF1 Monoclonal Antibody (5E2D7)

2 Published Figures

11 References

View all (11) ATPIF1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (24)
Published Figures (2)

ATPIF1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 26

ATPIF1 Antibody (A-21355) in ICC/IF

CAKI cells were plated on coverslips overnight. The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (Product # R37602) according to protocol. Following suppression of background binding using Image-iT® FX Signal enhancer (Product # R37107), cells were incubated with 3 µg/mL anti-ATP synthase subunit IF1 (Product # A-21355) for labeling of mitochondria for 30 min at room temperature and washed three times with dPBS, followed by Alexa Fluor® 594 goat anti-rabbit IgG antibody ReadyProbes® reagent ... View More

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Product Details

A-21355

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-5 µg/mL

View 5 publications 5 publications

in situ PLA (PLA)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Bovine, Dog, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5E2D7

Immunogen

Recombinant IF1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2535841

Product Specific Information

The antibody was produced in vitro using hybridomas grown in serum-free medium, then purified by biochemical fractionation, and is near homogeneity as judged by SDS-PAGE.

This antibody recognizes bands at ~10 and ~18 kDa on western analysis.

Target Information

Complex V, also called F1F0 ATPase or ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP + phosphate to ATP, in oxidative phosphorylation, and as a proton pumping ATPase. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The enzyme was thought to be localized exclusively to mitochondria; however, it has recently been identified on the plasma membrane of several cell types including hepatocytes where it functions as the HDL receptor, on endothelial cells where it may act as the angiostatin receptor, and on the surface of cancer cells. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits; while the F0 consists of a, b, and c subunits. The enzyme in mammals is composed of 17 subunits, five of which make up the easily detached F1. The remainder subunits are components of two stalk domains and the proton pumping F0 part of the machinery. Two of the subunits of the F0 part are encoded on mitochondrial DNA while the other subunits are nuclearly encoded. Mutations in the mitochondrial-encoded subunits of ATP synthase (Complex V) cause OXPHOS disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration.

Cell

Tracy TE,Madero-Pérez J,Swaney DL,Chang TS,Moritz M,Konrad C,Ward ME,Stevenson E,Hüttenhain R,Kauwe G,Mercedes M,Sweetland-Martin L,Chen X,Mok SA,Wong MY,Telpoukhovskaia M,Min SW,Wang C,Sohn PD,Martin J,Zhou Y,Luo W,Trojanowski JQ,Lee VMY,Gong S,Manfredi G,Coppola G,Krogan NJ,Geschwind DH,Gan L

A-21355 was used in Proximity Ligation Assay (PLA) to demonstrate Tau’s role in neuronal function and disease and highlight potential therapeutic targets to block Tau-mediated pathogenesis.

Thu Feb 17 00:00:00 UTC 2022

GRP78 Overexpression Triggers PINK1-IP₃R-Mediated Neuroprotective Mitophagy.

Biomedicines

Leiva-Rodríguez T,Romeo-Guitart D,Herrando-Grabulosa M,Muñoz-Guardiola P,Polo M,Bañuls C,Petegnief V,Bosch A,Lizcano JM,Apostolova N,Forés J,Casas C

A-21355 was used in Immunohistochemistry (Frozen) to study how the overexpression of GRP78 promoted motoneuronal neuroprotection after root avulsion by carrying out a comparative unbiased proteomic analysis and pharmacological and genetic interventions.

Wed Aug 18 00:00:00 UTC 2021

GRP78 Overexpression Triggers PINK1-IP₃R-Mediated Neuroprotective Mitophagy.

Biomedicines

Leiva-Rodríguez T,Romeo-Guitart D,Herrando-Grabulosa M,Muñoz-Guardiola P,Polo M,Bañuls C,Petegnief V,Bosch A,Lizcano JM,Apostolova N,Forés J,Casas C

Wed Aug 18 00:00:00 UTC 2021

Mitochondrial Proteome of Affected Glutamatergic Neurons in a Mouse Model of Leigh Syndrome.

Frontiers in cell and developmental biology

Gella A,Prada-Dacasa P,Carrascal M,Urpi A,González-Torres M,Abian J,Sanz E,Quintana A

A-21355 was used in Western Blotting to show a marked reduction of complex I N-module subunit abundance and an increase in the levels of the assembly factor NDUFA2.

Mon Sep 28 00:00:00 UTC 2020

Mitochondrial Proteome of Affected Glutamatergic Neurons in a Mouse Model of Leigh Syndrome.

Frontiers in cell and developmental biology

Gella A,Prada-Dacasa P,Carrascal M,Urpi A,González-Torres M,Abian J,Sanz E,Quintana A

A-21355 was used in Western Blotting to show a marked reduction of complex I N-module subunit abundance and an increase in the levels of the assembly factor NDUFA2.

Mon Sep 28 00:00:00 UTC 2020

OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation.

Nature communications

Sharanek A,Burban A,Laaper M,Heckel E,Joyal JS,Soleimani VD,Jahani-Asl A

A-21355 was used in Immunocytochemistry-Immunofluorescence to show that suppression of OSMR improves glioblastoma response to IR and prolongs lifespan.

Mon Aug 17 00:00:00 UTC 2020

OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation.

Nature communications

Sharanek A,Burban A,Laaper M,Heckel E,Joyal JS,Soleimani VD,Jahani-Asl A

A-21355 was used in Immunocytochemistry-Immunofluorescence to show that suppression of OSMR improves glioblastoma response to IR and prolongs lifespan.

Mon Aug 17 00:00:00 UTC 2020

Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release.

JCI insight

Obata Y,Kita S,Koyama Y,Fukuda S,Takeda H,Takahashi M,Fujishima Y,Nagao H,Masuda S,Tanaka Y,Nakamura Y,Nishizawa H,Funahashi T,Ranscht B,Izumi Y,Bamba T,Fukusaki E,Hanayama R,Shimada S,Maeda N,Shimomura I

A-21355 was used in Immunocytochemistry-immunoflourescence to demonstrate that the adiponectin/T-cadherin system enhances exosome biogenesis and secretion, leading to the decrease of cellular ceramides.

Thu Apr 19 00:00:00 UTC 2018

Endosome-mitochondria interactions are modulated by iron release from transferrin.

The Journal of cell biology

Das A,Nag S,Mason AB,Barroso MM

A21355 was used in immunocytochemistry to study the interactions between transferrin-containing endosomes and mitochondria in epithelial cells

Mon Sep 26 00:00:00 UTC 2016

GOLPH3 drives cell migration by promoting Golgi reorientation and directional trafficking to the leading edge.

Molecular biology of the cell

Xing M,Peterman MC,Davis RL,Oegema K,Shiau AK,Field SJ

A21355 was used in immunocytochemistry to investigate the role of the phosphatidylinositol-4-phosphate/GOLPH3/myosin 18A/F-actin pathway to directional cell migration

Thu Dec 01 00:00:00 UTC 2016

IF1, a natural inhibitor of mitochondrial ATP synthase, is not essential for the normal growth and breeding of mice.

Bioscience reports

Nakamura J,Fujikawa M,Yoshida M

A-21355 was used in Immunocytochemistry-immunoflourescence to establish that IF1 is not an essential protein for mice despite its ubiquitous presence in eukaryotes.

Tue Sep 17 00:00:00 UTC 2013

Equine chorionic gonadotropin alters luteal cell morphologic features related to progesterone synthesis.

Theriogenology

Rigoglio NN,Fátima LA,Hanassaka JY,Pinto GL,Machado AS,Gimenes LU,Baruselli PS,Rennó FP,Moura CE,Watanabe IS,Papa PC

A-21355 was used in immunohistochemistry (paraffin) to test if eCG alters the content of luteal cells and mitochondria related to hormone production.

Fri Mar 01 00:00:00 UTC 2013

Arenavirus infection induces discrete cytosolic structures for RNA replication.

Journal of virology

Baird NL,York J,Nunberg JH

A-21355 was used in western blot to identify and study cytosolic structures involved in arenavirus replication and transcription.

Mon Oct 01 00:00:00 UTC 2012

Mitochondrial DNA toxicity compromises mitochondrial dynamics and induces hippocampal antioxidant defenses.

DNA repair

Lauritzen KH,Cheng C,Wiksen H,Bergersen LH,Klungland A

A-21355 was used in immunohistochemistry - paraffin section to investigate the effects of mitochondrial DNA damage on hippocampal neurons.

Fri Jun 10 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: ATP synthase F1 subunit epsilon; ATP synthase inhibitor protein; ATPase inhibitor (rat mitochondrial IF1 protein); ATPase inhibitor protein; ATPase inhibitor, mitochondrial; IF(1); IF1; Inhibitor of F(1)F(o)-ATPase

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Gene Aliases: ATP5IF1; ATPI; ATPIF1; ATPIP; BOS_2311; IF(1); If1; IF1PA; IP

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UniProt ID: (Human) Q9UII2, (Bovine) P01096, (Mouse) O35143, (Rat) Q03344

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Entrez Gene ID: (Human) 93974, (Bovine) 327699, (Mouse) 11983, (Rat) 25392

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Function(s)

enzyme inhibitor activity calmodulin binding 5-formyltetrahydrofolate cyclo-ligase activity ATPase inhibitor activity protein homodimerization activity angiostatin binding ATPase binding protein binding

Process(es)

angiogenesis negative regulation of endothelial cell proliferation generation of precursor metabolites and energy heme biosynthetic process folic acid-containing compound biosynthetic process erythrocyte differentiation negative regulation of ATPase activity tetrahydrofolate interconversion protein homooligomerization protein homotetramerization negative regulation of hydrolase activity mitochondrial depolarization reactive oxygen species metabolic process mitophagy in response to mitochondrial depolarization positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway positive regulation of proteolysis involved in cellular protein catabolic process regulation of protein targeting to mitochondrion regulation of ATP metabolic process negative regulation of nucleotide metabolic process regulation of gene expression regulation of mitophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATPIF1 Monoclonal Antibody (5E2D7)

ATPIF1 Antibody (A-21355) in ICC/IF

Product Details

A-21355

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

in situ PLA (PLA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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