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FIGURE: 1 / 1
ATR Antibody (600-401-A30) in WB
Western blot using Rocklands Anti-ATR antibody. Lane 1: HeLa cell nuclear extract (p/n W09-001-367). Lane 2: HeLa cell nuclear extract pre-incubated with the immunizing peptide (50 µg peptide for 1 h at room temperature followed by centrifugation). Goat serum was used at 5% for blocking. Primary antibody: Anti-ATR at 1:1,400 dilution. The arrowhead corresponds to ~301kDa ATR when compared to MW markers (Lane M).
Product Details
600-401-A30
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Fish,
Human,
Mouse,
Non-human primate,
Rat,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.
Conjugate
Form
Liquid
Concentration
1.28 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human ATR protein. A BLAST analysis was used to suggest cross-reactivity with ATR from human, mouse, rat, monkey, dog, fish and Xenopus sources based on a 100% homology with the immunizing sequence. Cross-reactivity with ATR from other sources has not been determined.
Target Information
The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2310008J16Rik; 2810405N18Rik; ataxia telangiectasia and Rad3 related; Ataxia telangiectasia and Rad3-related protein; ATR serine/threonine kinase; EC 2.7.11.1; FRAP-related protein 1; FRAP-related protein-1; kinase ATR; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; Serine/threonine-protein kinase ATR; TEM8
Gene Aliases: ATR; FCTCS; FRP1; MEC1; SCKL; SCKL1
UniProt ID: (Human) Q13535
Entrez Gene ID: (Human) 545, (Dog) 100856315, (Mouse) 245000, (Rat) 685055
DNA binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
MutLalpha complex binding
MutSalpha complex binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
phosphotransferase activity, alcohol group as acceptor
protein dimerization activity
molecular_function
DNA damage checkpoint
double-strand break repair via homologous recombination
DNA repair
protein phosphorylation
cellular response to DNA damage stimulus
negative regulation of DNA replication
phosphorylation
peptidyl-serine phosphorylation
cellular response to UV
regulation of protein binding
positive regulation of DNA damage response, signal transduction by p53 class mediator
protein autophosphorylation
cellular response to gamma radiation
replicative senescence
DNA replication
cell cycle
multicellular organism development
positive regulation of telomere maintenance via telomerase
interstrand cross-link repair
response to drug
protein localization to chromosome, telomeric region
establishment of RNA localization to telomere
establishment of macromolecular complex localization to telomere
regulation of cellular response to heat
regulation of signal transduction by p53 class mediator
positive regulation of telomerase catalytic core complex assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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