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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • ATR Antibodies

          Invitrogen

          ATR Polyclonal Antibody

          Advanced Verification
          4 Published Figures
          20 References
          View all (29) ATR antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite ATR Polyclonal Antibody

          • Antibody Testing Data (2)
          • Published Figures (4)
          • Advanced Verification (2)
          ATR Antibody in Western Blot (WB)
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          ATR Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          ATR Antibody (PA1-450) in WB

          Western blot analysis of ATR was performed with 10 µg of HeLa cells transfected with Transfection Reagent alone (Lane 1), 100nM Non-Targeting control siRNA (Lane 2), or 100nM siRNA against ATR (Lane 3). Proteins were resolved using a NuPAGE® Novex 4-12% Bis-Tris Gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and a protein size ladder. Proteins were wet transferred to a Pierce Nitrocellulose Membrane (Product # 88025) OR Pierce PVDF Membrane (Product # 88518) and blocked with Pierce Starting Block T20 ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          ATR Antibody in Western Blot (WB)
          ATR Antibody in Western Blot (WB)
          ATR Antibody in Western Blot (WB)
          ATR Antibody in Western Blot (WB)
          ATR Antibody in Western Blot, Immunoprecipitation (WB, IP)
          ATR Antibody in Western Blot, Immunoprecipitation (WB, IP)
          ATR Antibody
          ATR Antibody

          Product Details

          PA1-450

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 17 publications 17 publications

          Immunoprecipitation (IP)

          Assay-dependent
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Fusion protein containing residues 400-460 of the human ATR protein.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          4 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_325822

          Product Specific Information

          PA1-450 detects ATR (Ataxia Telangiectasia Mutated (ATM) and Rad3-related protein) from human cells.

          PA1-450 has been successfully used in Western blot immunofluoresence and immunoprecipitation procedures. By Western blot, this antibody detects an ~305 kDa protein representing ATR in lysate from UV irradiated K562 cells.

          PA1-450 antigen is a fusion protein corresponding to amino acid residues 400-460 from human ATR.

          Target Information

          The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 2310008J16Rik; 2810405N18Rik; Ataxia telangiectasia and Rad3-related protein; EC 2.7.11.1; FRAP-related protein 1; FRAP-related protein-1; kinase ATR; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; Serine/threonine-protein kinase ATR; TEM8

          View more View less

          Gene Aliases: ATR; FCTCS; FRP1; MEC1; SCKL; SCKL1

          View more View less

          UniProt ID: (Human) Q13535

          View more View less

          Entrez Gene ID: (Human) 545

          View more View less

          Function(s)
          DNA binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding MutLalpha complex binding MutSalpha complex binding
          Process(es)
          DNA damage checkpoint DNA replication DNA repair cellular response to DNA damage stimulus cell cycle multicellular organism development negative regulation of DNA replication peptidyl-serine phosphorylation positive regulation of telomere maintenance via telomerase cellular response to UV interstrand cross-link repair response to drug positive regulation of DNA damage response, signal transduction by p53 class mediator protein autophosphorylation protein localization to chromosome, telomeric region cellular response to gamma radiation replicative senescence establishment of RNA localization to telomere establishment of macromolecular complex localization to telomere regulation of cellular response to heat regulation of signal transduction by p53 class mediator positive regulation of telomerase catalytic core complex assembly
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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