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Invitrogen
ATXN7L3 Monoclonal Antibody (2ATX-2B1)
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FIGURE: 1 / 2
ATXN7L3 Antibody (MA3-084) in ICC/IF
Immunofluorescent analysis of ATXN7L3 (green) in Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ATXN7L3 mouse monoclonal antibody (Product # MA3-084) at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal™ Secondary Antibody, Alexa Fluor 488 conjugate (Product # A2817... View More
Product Details
MA3-084
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2ATX-2B1
Immunogen
Synthetic Peptide coupled to Ovalbumin corresponding to aa 278 to aa 296 of human ataxin 7L3
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633329
Product Specific Information
MA3-084 detects Ataxin 7L3 from human and mouse samples.
MA3-084 has been successfully used in Western blot, immunofluorescence, and ELISA applications.
Target Information
Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B (PubMed:18206972, PubMed:21746879). The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. Within the complex, it is required to recruit USP22 and ENY2 into the SAGA complex (PubMed:18206972). Regulates H2B monoubiquitination (H2Bub1) levels. Affects subcellular distribution of ENY2, USP22 and ATXN7L3B (PubMed:27601583). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT7L3; ataxin 7-like 3; Ataxin-7-like protein 3; ATXN7L3; DKFZp761G2113; SAGA-associated factor 11 homolog
Gene Aliases: Atxn7l3; E030022H21Rik
UniProt ID: (Mouse) A2AWT3
Entrez Gene ID: (Human) 56970, (Mouse) 217218
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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