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Bethyl Laboratories
AU1 Tag Polyclonal Antibody
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AU1 Tag Antibody (A190-125A) in WB
Detection of AU1-tagged protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-AU1 antibody (Product # A190-125A) used for WB at 0.04 µg/mL (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
Product Details
A190-125A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
AU1 (DTYRYI) conjugated to KLH.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 6.8 to 7.4
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Rabbits were immunized with AU1 (DTYRYI) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Target Information
AU1 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized by immunochemical methods. The recognized AU1 sequence represents the amino acid sequence DTYRYI, derived from the major capsid protein of bovine papillomavirus-1 (BPV-1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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