Invitrogen

AU1 Tag Polyclonal Antibody

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AU1 Tag Antibody (PA1-26547) in WB

Western Blot analysis of 200, 100, and 50 ng of E. coli lysate containing tagged fusion protein using AU1 Tag Polyclonal Antibody (Product # PA1-26547). Dilution: 1:5,000.

AU1 Tag Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

PA1-26547

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 2 publications 2 publications

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:400

ELISA (ELISA)

1:1,000-1:30,000

Immunoprecipitation (IP)

1-4 µg/mg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AU1 (DTYRYI) conjugated to KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_779329

Product Specific Information

PA1-26547 detects AU1 tag.

Target Information

AU1 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized by immunochemical methods. The recognized AU1 sequence represents the amino acid sequence DTYRYI, derived from the major capsid protein of bovine papillomavirus-1 (BPV-1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Histidine Residues Are Responsible for Bidirectional Effects of Zinc on Acid-Sensing Ion Channel 1a/3 Heteromeric Channels.

Biomolecules

Jiang Q,Peterson AM,Chu Y,Yao X,Zha XM,Chu XP

PA1-26547 was used in Western Blot, Immunoprecipitation to suggest that histidine residues in the extracellular domain of heteromeric ASIC1a/3 channels are critical for zinc-mediated effects.

Wed Sep 02 00:00:00 UTC 2020

Histidine Residues Are Responsible for Bidirectional Effects of Zinc on Acid-Sensing Ion Channel 1a/3 Heteromeric Channels.

Biomolecules

Jiang Q,Peterson AM,Chu Y,Yao X,Zha XM,Chu XP

PA1-26547 was used in Western Blot, Immunoprecipitation to suggest that histidine residues in the extracellular domain of heteromeric ASIC1a/3 channels are critical for zinc-mediated effects.

Wed Sep 02 00:00:00 UTC 2020

Transforming Growth Factor β1-induced Apoptosis in Podocytes via the Extracellular Signal-regulated Kinase-Mammalian Target of Rapamycin Complex 1-NADPH Oxidase 4 Axis.

The Journal of biological chemistry

Das R,Xu S,Nguyen TT,Quan X,Choi SK,Kim SJ,Lee EY,Cha SK,Park KS

PA1-26547 was used in western blot to characterize TGF Beta1-induced apoptosis in podocytes via extracellular signal-regulated kinase-mammalian target of rapamycin complex 1-NADPH oxidase 4 axis

Fri Dec 25 00:00:00 UTC 2015

Transforming Growth Factor β1-induced Apoptosis in Podocytes via the Extracellular Signal-regulated Kinase-Mammalian Target of Rapamycin Complex 1-NADPH Oxidase 4 Axis.

The Journal of biological chemistry

Das R,Xu S,Nguyen TT,Quan X,Choi SK,Kim SJ,Lee EY,Cha SK,Park KS

PA1-26547 was used in western blot to characterize TGF Beta1-induced apoptosis in podocytes via extracellular signal-regulated kinase-mammalian target of rapamycin complex 1-NADPH oxidase 4 axis

Fri Dec 25 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: AU1 Epitope Tag

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AU1 Tag Polyclonal Antibody

AU1 Tag Antibody (PA1-26547) in WB

Product Details

PA1-26547

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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