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AWAT1 Antibody (PA5-103186) in ICC/IF
Immunofluorescent analysis of AWAT1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and AWAT1 polyclonal antibody (Product # PA5-103186) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-103186
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human AWAT1(Accession Q58HT5), corresponding to amino acid residues L99-Y149.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852554
Product Specific Information
Antibody detects endogenous levels of total AWAT1.
Target Information
The protein encoded by this gene belongs to the diacylglycerol acyltransferase family. It esterifies long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that this enzyme plays a central role in lipid metabolism in skin. Consistent with this observation, this protein is predominantly expressed in the sebaceous gland of the skin. [provided by RefSeq, Sep 2009].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acyl-CoA wax alcohol acyltransferase 1; diacyl-glycerol acyltransferase 2; Diacylglycerol acyltransferase 2; diacylglycerol O-acyltransferase 2-like 3; Diacylglycerol O-acyltransferase 2-like protein 3; Long-chain-alcohol O-fatty-acyltransferase 1; wax synthase 1
Gene Aliases: AWAT1; DGA2; DGAT2L3
UniProt ID: (Human) Q58HT5, (Mouse) A2ADU9
Entrez Gene ID: (Human) 158833, (Rat) 679520, (Mouse) 245533
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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