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          • Primary Antibodies ›
          • CBP/p300 Antibodies

          Invitrogen

          Acetyl-CBP/p300 (Lys1499, Lys1535) Polyclonal Antibody

          1 Reference
          View all (2) CBP/p300 antibodies

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          Cite Acetyl-CBP/p300 (Lys1499, Lys1535) Polyclonal Antibody

          Product Details

          PA5-16185

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          1:50
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Non-human primate

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic acetylated peptide corresponding to residues surrounding Lys1535 of human CBP
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          45 µg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10979782

          Product Specific Information

          It is not recommended to aliquot this antibody.

          Target Information

          This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CREB-binding protein; E1A-associated protein p300; E1A-binding protein, 300kD; Histone acetyltransferase p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; Histone lysine acetyltransferase CREBBP; p300 HAT; Protein 2-hydroxyisobutyryltransferase p300; Protein lactyltransferas CREBBP; Protein lactyltransferas p300; Protein propionyltransferase p300; Protein-lysine acetyltransferase CREBBP

          View more View less

          Gene Aliases: A430090G16; A730011L11; AW558298; CBP; CBP/p300; CREBBP; EP300; KAT3A; KAT3B; P300; p300 HAT; p300/CBP; RSTS; RSTS2

          View more View less

          UniProt ID: (Human) Q92793, (Human) Q09472, (Mouse) B2RWS6

          View more View less

          Entrez Gene ID: (Human) 1387, (Mouse) 12914, (Human) 2033, (Mouse) 328572

          View more View less

          Function(s)
          core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity transcriptional repressor activity, RNA polymerase II transcription factor binding p53 binding chromatin binding damaged DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity histone acetyltransferase activity signal transducer activity protein binding transcription factor binding zinc ion binding acetyltransferase activity peptide N-acetyltransferase activity peroxisome proliferator activated receptor binding MRF binding SMAD binding RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription cofactor activity transferase activity transferase activity, transferring acyl groups activating transcription factor binding metal ion binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter binding lysine N-acetyltransferase activity, acting on acetyl phosphate as donor beta-catenin binding protein C-terminus binding chromatin DNA binding protein complex binding nuclear hormone receptor binding glucocorticoid receptor binding bHLH transcription factor binding androgen receptor binding mitogen-activated protein kinase binding NF-kappaB binding pre-mRNA intronic binding protein antigen binding core promoter proximal region DNA binding antigen binding protein kinase binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein acetylation cell proliferation histone acetylation N-terminal peptidyl-lysine acetylation germ-line stem cell population maintenance cellular response to UV positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of G1/S transition of mitotic cell cycle response to hypoxia somitogenesis liver development positive regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway transcription-coupled nucleotide-excision repair internal protein amino acid acetylation apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response Notch signaling pathway nervous system development heart development skeletal muscle tissue development memory circadian rhythm organ morphogenesis regulation of autophagy positive regulation of glycoprotein biosynthetic process macrophage derived foam cell differentiation positive regulation of cell death positive regulation of muscle atrophy viral process internal peptidyl-lysine acetylation B cell differentiation platelet formation lung development response to cobalt ion positive regulation of protein binding positive regulation of type I interferon production positive regulation of collagen biosynthetic process positive regulation of protein import into nucleus, translocation response to tumor necrosis factor positive regulation of histone acetylation cellular response to drug megakaryocyte development cellular response to trichostatin A intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of DNA binding protein kinase B signaling response to estrogen positive regulation by host of viral transcription histone H3 acetylation histone H4 acetylation histone H2B acetylation fat cell differentiation response to ethanol positive regulation of translation positive regulation of axon extension positive regulation of cell size positive regulation of gene expression, epigenetic positive regulation of proteolysis digestive tract development positive regulation of protein secretion protein stabilization response to calcium ion regulation of cell cycle regulation of angiotensin metabolic process positive regulation of sarcomere organization regulation of androgen receptor signaling pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia protein-DNA complex assembly cellular response to hydrogen peroxide response to fatty acid cellular response to retinoic acid cellular response to cAMP cellular response to glucose stimulus cellular response to mineralocorticoid stimulus cellular response to dexamethasone stimulus regulation of tubulin deacetylation regulation of cellular response to heat regulation of signal transduction by p53 class mediator beta-catenin-TCF complex assembly cellular response to nerve growth factor stimulus negative regulation of miRNA metabolic process cell cycle response to glucose positive regulation of gene expression cell differentiation positive regulation of cell growth negative regulation of cellular metabolic process positive regulation of cellular metabolic process response to drug cartilage development negative regulation of cell death response to dexamethasone positive regulation of protein acetylation regulation of nucleic acid-templated transcription transcription initiation from RNA polymerase II promoter protein complex assembly signal transduction regulation of smoothened signaling pathway homeostatic process embryonic digit morphogenesis regulation of apoptotic process cellular lipid metabolic process positive regulation of cell adhesion molecule production response to interleukin-1 cell-cell adhesion positive regulation of NIK/NF-kappaB signaling
          It has to be done as per old AB suggested Products section.
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