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Invitrogen
Actin Polyclonal Antibody
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Actin Antibody (PA5-78716) in IHC (P)
Immunohistochemistry (Paraffin) analysis of Actin in paraffin-embedded section of human colorectal adenocarcinoma tissue using Actin Polyclonal Antibody (Product # PA5-78716). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL diluti... View More
Product Details
PA5-78716
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (359-377aa ITKQEYDEAGPSIVHRKCF).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745832
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human Hela whole cell, human HepG2 whole cell, human 293T whole cell, human A431 whole cell, human U87 whole cell, human U937 whole cell, human K562 whole cell, rat heart tissue, rat brain tissue, rat kidney tissue, mouse heart tissue, mouse brain tissue, mouse spleen tissue, mouse kidney tissue. IHC: human breast cancer tissue, human colorectal adenocarcinoma tissue, human placenta tissue, human tonsil tissue, mouse heart tissue, rat heart tissue.
Target Information
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 2; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; Atcb; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; smooth muscle alpha-actin; Smooth muscle gamma-actin
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; ASD5; ASMA; beta-actin; BRWS1; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E430023M04Rik; E51; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMGA; VSCM
structural constituent of cytoskeleton
protein binding
ATP binding
myosin binding
ADP binding
protein kinase binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
kinesin binding
Tat protein binding
nucleosomal DNA binding
identical protein binding
nitric-oxide synthase binding
ATPase activity
ubiquitin protein ligase binding
nucleotide binding
molecular_function
apoptotic process
positive regulation of gene expression
actin filament-based movement
muscle filament sliding
skeletal muscle thin filament assembly
actomyosin structure organization
actin-myosin filament sliding
response to drug
negative regulation of apoptotic process
response to ethanol
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
heart contraction
cardiac muscle contraction
mesenchyme migration
retina homeostasis
movement of cell or subcellular component
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
sarcomere organization
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
muscle contraction
regulation of blood pressure
response to virus
vascular smooth muscle contraction
glomerular mesangial cell development
skeletal muscle fiber development
axonogenesis
substantia nigra development
ATP-dependent chromatin remodeling
retina development in camera-type eye
cellular response to electrical stimulus
metabolic process
actin-mediated cell contraction
response to mechanical stimulus
response to extracellular stimulus
response to lithium ion
cell growth
skeletal muscle fiber adaptation
response to steroid hormone
biological_process
cytoskeleton organization
positive regulation of gene expression, epigenetic
response to calcium ion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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