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Invitrogen
Actin Monoclonal Antibody (A2E1)
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Actin Antibody (MA5-42536) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded Human fetal skeletal muscle tissue using Actin Monoclonal Antibody (Product # MA5-42536). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the Actin antibody at ... View More
Product Details
MA5-42536
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
A2E1
Immunogen
Synthetic peptide corresponding to N terminal of Human Alpha actin (smooth muscle, skeletal muscle, cardiac muscle)
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911677
Product Specific Information
Positive Control: Rat heart tissue lysate, mouse smooth muscle tissue lysate, human skeletal muscle tissue lysate.
Subcellular Location: Cytoplasm, Cytoskeleton
Target Information
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 2; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; Atcb; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; smooth muscle alpha-actin; Smooth muscle gamma-actin
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; ASD5; ASMA; beta-actin; BRWS1; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E430023M04Rik; E51; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMGA; VSCM
structural constituent of cytoskeleton
protein binding
ATP binding
myosin binding
ADP binding
protein kinase binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
kinesin binding
Tat protein binding
nucleosomal DNA binding
identical protein binding
nitric-oxide synthase binding
ATPase activity
ubiquitin protein ligase binding
nucleotide binding
molecular_function
apoptotic process
positive regulation of gene expression
actin filament-based movement
muscle filament sliding
skeletal muscle thin filament assembly
actomyosin structure organization
actin-myosin filament sliding
response to drug
negative regulation of apoptotic process
response to ethanol
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
heart contraction
cardiac muscle contraction
mesenchyme migration
retina homeostasis
movement of cell or subcellular component
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
sarcomere organization
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
muscle contraction
regulation of blood pressure
response to virus
vascular smooth muscle contraction
glomerular mesangial cell development
skeletal muscle fiber development
axonogenesis
substantia nigra development
ATP-dependent chromatin remodeling
retina development in camera-type eye
cellular response to electrical stimulus
metabolic process
actin-mediated cell contraction
response to mechanical stimulus
response to extracellular stimulus
response to lithium ion
cell growth
skeletal muscle fiber adaptation
response to steroid hormone
biological_process
cytoskeleton organization
positive regulation of gene expression, epigenetic
response to calcium ion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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