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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Actin Antibodies

          Invitrogen

          Actin Monoclonal Antibody (mAbGEa)

          Advanced Verification
          65 Published Figures
          134 References
          View all (21) Actin antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Actin Monoclonal Antibody (mAbGEa)

          • Antibody Testing Data (8)
          • Published Figures (65)
          • Advanced Verification (2)
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 75

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          Actin Antibody (MA1-744) in ICC/IF

          Immunofluorescence analysis of Actin, cytoplasmic 1 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Pl... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Actin Antibody in Immunocytochemistry (ICC/IF)
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Actin Antibody in Immunocytochemistry (ICC/IF)
          Actin Antibody in Immunohistochemistry (IHC)
          Actin Antibody in Immunohistochemistry (IHC)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Western Blot (WB)
          Actin Antibody in Immunoprecipitation (IP)
          Actin Antibody
          Actin Antibody

          Product Details

          MA1-744

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:10,000
          View 119 publications 119 publications

          Immunohistochemistry (IHC)

          -
          View 4 publications 4 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100
          -

          Immunocytochemistry (ICC/IF)

          1:10-1:100
          View 4 publications 4 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          Assay-dependent
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Bovine, Fruit fly, Human, Mouse, Sheep, Plant, Rat, Xenopus, Yeast, Zebrafish

          Published species

          Bacteria, Bovine, Fruit fly, Goat, Human, Insect, Mouse, Pig, Plant, Rat, Xenopus, Yeast

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          mAbGEa

          Immunogen

          Purified Arabidopsis actin protein.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          ascites

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2223496

          Product Specific Information

          MA1-744 detects actin protein in human, mouse, rat, bovine, sheep, drosophila, zebra fish (Danio rerio), yeast, arabidopsis and xenopus samples. This antibody has shown cross-reactivity with actin 1, 2, 3, 4, 7, 8, 11 and 12.

          MA1-744 has successfully been used in Western blot and ELISA procedures. By Western blot, this antibody detects a 45 kDa protein representing actin.

          The MA1-744 immunogen is purified actin protein from Arabidopsis.

          Target Information

          Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 5C actin; Act5C-PA; Act5C-PB; Act5C-PC; Act5C-PD; Act5C-PE; actin; actin 5 C; actin 5C; actin A1; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; Actin-5C; actin-like protein; actin5C; alpha 1 skeletal muscle actin; alpha actin 2; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; anon-fast-evolving-2D2; Atcb; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cellular cytoskeletal beta-actin; CG4027-PA; CG4027-PB; CG4027-PC; CG4027-PD; CG4027-PE; Cytoplasmic beta-actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; Dm-actin5C; epididymis luminal protein 176; F-actin; G-actin; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; sma; smooth muscle alpha-actin; Smooth muscle gamma-actin

          View more View less

          Gene Aliases: 0610041G09Rik; A; a-SMA; A4V404_DROME; AA959943; AAT6; Ac5C; ACT; act 5C; Act-4; Act-5C; ACT1_DROME; Act4; Act5; ACT5C; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actin; Actin/BAP47; Actin5C; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; anon-EST:fe2D2; ASD5; ASMA; BAP47; beta-actin; beta-actin/Bap47; BOS_10523; BOS_11389; BOS_19345; BOS_22932; BOS_23286; BOS_23966; BRWS1; BRWS2; CFTD; CFTD1; CFTDM; CG4027; chrX:5748184..5748304; CMD1R; CMH11; cyt5C; DFNA20; DFNA26; Dmel\CG4027; Dmel_CG4027; E430023M04Rik; E51; GIG46; HEL-176; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0177; l(1)G0245; l(1)G0330; l(1)G0420; l(1)G0486; LVNC4; M32055; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMGA; T11; VSCM; wu:fb63d03; XELAEV_18029136mg; XELAEV_18045052mg

          View more View less

          UniProt ID: (Human) P68133, (Bovine) P68138, (Bovine) P62739, (Human) P62736, (Bovine) P60712, (Human) P60709, (Sheep) P60713, (Human) P68032, (Bovine) Q3ZC07, (Human) P63261, (Bovine) P63258, (Bovine) Q5E9B5, (Human) P63267, (Fruit fly) A4V404, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P60711, (Mouse) P60710, (Rat) P68035, (Mouse) P68033, (Mouse) P63260, (Rat) P63259, (Mouse) P63268, (Rat) P63269, (Xenopus) O93400

          View more View less

          Entrez Gene ID: (Human) 58, (Bovine) 281592, (Sheep) 101107662, (Bovine) 515610, (Human) 59, (Sheep) 101121051, (Bovine) 280979, (Human) 60, (Sheep) 443052, (Human) 70, (Bovine) 533219, (Sheep) 101111554, (Human) 71, (Bovine) 404122, (Sheep) 443340, (Sheep) 101114256, (Bovine) 281595, (Human) 72, (Fruit fly) 31521, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rat) 81822, (Mouse) 11461, (Rat) 29275, (Mouse) 11464, (Mouse) 11465, (Rat) 287876, (Mouse) 11468, (Rat) 25365, (Xenopus) 432314, (Zebrafish) 322509, (Xenopus) 398459

          View more View less

          Function(s)
          structural constituent of cytoskeleton protein binding ATP binding myosin binding ADP binding protein kinase binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding ATPase activity ubiquitin protein ligase binding nucleotide binding molecular_function
          Process(es)
          retina homeostasis movement of cell or subcellular component cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis sarcomere organization vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation protein folding chaperone mediated protein folding independent of cofactor skeletal muscle thin filament assembly skeletal muscle fiber development axonogenesis substantia nigra development ATP-dependent chromatin remodeling retina development in camera-type eye cellular response to electrical stimulus metabolic process actin filament-based movement actomyosin structure organization actin-myosin filament sliding response to drug negative regulation of apoptotic process response to ethanol cardiac myofibril assembly cardiac muscle tissue morphogenesis heart contraction cardiac muscle contraction actin-mediated cell contraction blood circulation positive regulation of gene expression mesenchyme migration muscle contraction response to mechanical stimulus response to extracellular stimulus response to lithium ion cell growth muscle filament sliding skeletal muscle fiber adaptation response to steroid hormone cytoskeleton organization positive regulation of gene expression, epigenetic apoptotic process regulation of blood pressure response to virus vascular smooth muscle contraction glomerular mesangial cell development mitotic cytokinesis inter-male aggressive behavior chromatin remodeling phagocytosis sperm individualization mushroom body development maintenance of protein location in cell tube formation biological_process response to calcium ion
          It has to be done as per old AB suggested Products section.
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