Invitrogen

Actin (muscle) Monoclonal Antibody (HHF35), Alexa Fluor™ 488, eBioscience™

1 Published Figure

2 References

View all (17) Actin Muscle antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

Actin (muscle) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

Actin (muscle) Antibody (53-6496-82) in ICC/IF

Immunocytochemistry on fixed and permeabilized C2C12 cells stained with 30 µg/mL Mouse IgG1 Alexa Fluor® 488 Isotype Control (left) or 30 µg/mL Anti-Actin (muscle) Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

Actin (muscle) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

53-6496-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

30 µg/mL

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Guinea pig, Human, Mouse, Non-human primate, Pig, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HHF35

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2574433

Product Specific Information

Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42 kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.

The HHF35 antibody recognizes human, mouse, rat, bovine, canine and non-human primate actin.

Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This HHF35 antibody has been tested by immunocytochemistry and can be used at less than or equal to 30 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Adult Pgf^-/- mice behaviour and neuroanatomy are altered by neonatal treatment with recombinant placental growth factor.

Scientific reports

Kay VR,Cahill LS,Hanif A,Sled JG,Carmeliet P,Tayade C,Croy BA

53-6496 was used in Immunohistochemistry-wholemount to investigate whether developmentally asynchronous (i.e. neonatal) PGF supplementation alters the vascular, neuroanatomic and/or behavioral status of Pgf-/- mice at adulthood.

Wed Jun 26 00:00:00 UTC 2019

Restoration of Corneal Transparency by Mesenchymal Stem Cells.

Stem cell reports

Mittal SK,Omoto M,Amouzegar A,Sahu A,Rezazadeh A,Katikireddy KR,Shah DI,Sahu SK,Chauhan SK

53-6496 was used in Immunohistochemistry to demonstrate that mesenchymal stem cells can restore corneal transparency by secreting high levels of hepatocyte growth factor.

Tue Oct 11 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 1, skeletal muscle; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 2; actin, gamma 1; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha actin 1; alpha actin 2; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; beta-actin FE-3; cardiac actin; cardiac muscle alpha actin 1; Cell growth-inhibiting gene 46 protein; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; RP5-1068B5.2; skeletal alpha actin; smooth muscle alpha-actin; Smooth muscle gamma-actin; vascular smooth muscle alpha-actin

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Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; ACT-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; AL023024; alphaSMA; ASD5; ASMA; BOS_10523; BOS_11389; BOS_19345; BOS_23286; BOS_23966; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E51; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM; SMalphaA; SMGA; VSCM