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Adenylate Kinase 1 Antibody (PA5-28354) in IHC (P)
Adenylate Kinase 1 Polyclonal Antibody detects Adenylate kinase 1 protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. Adenylate Kinase 1 Polyclonal Antibody (Product # PA5-28354) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-28354
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Adenylate kinase 1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2545830
Product Specific Information
Recommended positive controls: A549, HCT116, mouse brown adipose tissue.
Predicted reactivity: Mouse (89%), Rat (90%), Xenopus laevis (80%), Dog (97%), Chicken (86%), Sheep (96%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adenylate kinase isoenzyme 1; Adenylate monophosphate kinase; AK 1; ATP-AMP transphosphorylase 1; ATP:AMP phosphotransferase; cytosolic adenylate kinase; Myokinase; testis secretory sperm binding protein Li 58j
Gene Aliases: Ak-1; AK1; B430205N08Rik; HTL-S-58j
UniProt ID: (Human) P00568, (Mouse) Q9R0Y5
Entrez Gene ID: (Human) 203, (Mouse) 11636
nucleoside diphosphate phosphorylation
ADP biosynthetic process
cell cycle arrest
nucleoside triphosphate biosynthetic process
nucleobase-containing small molecule interconversion
AMP metabolic process
ATP metabolic process
nucleobase-containing compound metabolic process
positive regulation of glucose transport
phosphorylation
inosine biosynthetic process
nucleotide phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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