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Invitrogen
Adiponectin Receptor 1 Recombinant Rabbit Monoclonal Antibody (SC69-04)
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FIGURE: 1 / 8
Adiponectin Receptor 1 Antibody (MA5-32249) in ICC/IF
Immunocytochemical analysis of Adiponectin Receptor 1 in HepG2 cells using a Adiponectin Receptor 1 Monoclonal antibody (Product # MA5-32249) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-32249
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SC69-04
Immunogen
Synthetic peptide within Human ADIPOR1 aa 5-54
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809535
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Adiponectin (also known as 30- kDa adipocyte complement-related protein; Acrp30) is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin in the blood are decreased under conditions of obesity, insulin resistance and type 2 diabetes. Administration of adiponectin causes glucose-lowering effects and ameliorates insulin resistance in mice. Conversely, adiponectin-deficient mice exhibit insulin resistance and diabetes. This insulin-sensitizing effect of adiponectin seems to be mediated by an increase in fatty-acid oxidation through activation of AMP kinase and PPAR-. Here we report the cloning of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) by expression cloning. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. These two adiponectin receptors are predicted to contain seven transmembrane domains, but are structurally and functionally distinct from G-protein-coupled receptors. Expression of AdipoR1/R2 or suppression of AdipoR1/R2 expression by small-interfering RNA supports our conclusion that they serve as receptors for globular and full-length adiponectin, and that they mediate increased AMP kinase and PPAR- ligand activities, as well as fatty-acid oxidation and glucose uptake by adiponectin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Adiponectin receptor protein 1; Progestin and adipoQ receptor family member 1; Progestin and adipoQ receptor family member I
Gene Aliases: 2810031L11Rik; ACDCR1; ADIPOR1; CGI-45; CGI45; PAQR1; Parq1; TESBP1A
UniProt ID: (Human) Q96A54, (Mouse) Q91VH1
Entrez Gene ID: (Human) 51094, (Rat) 289036, (Mouse) 72674
G-protein coupled receptor signaling pathway
hormone-mediated signaling pathway
negative regulation of epithelial cell migration
negative regulation of epithelial to mesenchymal transition
regulation of glucose metabolic process
regulation of lipid metabolic process
fatty acid oxidation
negative regulation of cell growth
leptin-mediated signaling pathway
adiponectin-activated signaling pathway
glucose homeostasis
negative regulation of JAK-STAT cascade
positive regulation of JAK-STAT cascade
positive regulation of insulin receptor signaling pathway
negative regulation of NIK/NF-kappaB signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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