Proteintech

Adiponectin receptor 1 Monoclonal Antibody (1F12B3)

Adiponectin receptor 1 Antibody in Western Blot (WB)

FIGURE: 1 / 2

Adiponectin receptor 1 Antibody (66619-1-IG) in WB

HeLa cells, L02 cells, and HepG2 cells were subjected to SDS PAGE followed by western blot with 66619-1-IG (Adiponectin receptor 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Product Image Gallery

Product Details

66619-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1F12B3

Immunogen

Adiponectin receptor 1 Fusion Protein Ag18187 (1-72 aa encoded by BC010743)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody was raised against 1-72 aa of human AdipoR1.

Immunogen sequence: MSSHKGSVV AQGNGAPASN READTVELAE LGPLLEEKGK RVIANPPKAE EEQTCPVPQE EEEEVRVLTL PLQ (1-72 aa encoded by BC010743)

Target Information

Adiponectin (also known as 30- kDa adipocyte complement-related protein; Acrp30) is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin in the blood are decreased under conditions of obesity, insulin resistance and type 2 diabetes. Administration of adiponectin causes glucose-lowering effects and ameliorates insulin resistance in mice. Conversely, adiponectin-deficient mice exhibit insulin resistance and diabetes. This insulin-sensitizing effect of adiponectin seems to be mediated by an increase in fatty-acid oxidation through activation of AMP kinase and PPAR-. Here we report the cloning of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) by expression cloning. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. These two adiponectin receptors are predicted to contain seven transmembrane domains, but are structurally and functionally distinct from G-protein-coupled receptors. Expression of AdipoR1/R2 or suppression of AdipoR1/R2 expression by small-interfering RNA supports our conclusion that they serve as receptors for globular and full-length adiponectin, and that they mediate increased AMP kinase and PPAR- ligand activities, as well as fatty-acid oxidation and glucose uptake by adiponectin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway.

Journal of agricultural and food chemistry

Tang C,Zhao H,Kong L,Meng F,Zhou L,Lu Z,Lu Y

Wed Jan 25 00:00:00 UTC 2023

Effect of dietary leucine supplementation on skeletal muscle fiber type transformation in weaning piglets.

Animal biotechnology

Chen X,Xiang L,Huang Z,Jia G,Liu G,Zhao H

Wed Jun 01 00:00:00 UTC 2022

Leucine regulates porcine muscle fiber type transformation via adiponectin signaling pathway.

Animal biotechnology

Xiang L,Huang Z,Chen X,Jia G,Liu G,Zhao H

Fri Apr 01 00:00:00 UTC 2022

A stem cell based in vitro model of NAFLD enables the analysis of patient specific individual metabolic adaptations in response to a high fat diet and AdipoRon interference.

Biology open

Graffmann N,Ncube A,Martins S,Fiszl AR,Reuther P,Bohndorf M,Wruck W,Beller M,Czekelius C,Adjaye J

Mon Jan 25 00:00:00 UTC 2021

Quercetin regulates skeletal muscle fiber type switching via adiponectin signaling.

Food & function

Chen X,Liang D,Huang Z,Jia G,Zhao H,Liu G

Sun Mar 21 00:00:00 UTC 2021

A flavonoid-rich Smilax china L. extract prevents obesity by upregulating the adiponectin-receptor/AMPK signalling pathway and modulating the gut microbiota in mice.

Food & function

Li X,Yang L,Li J,Lin L,Zheng G

Mon Jul 05 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: Adiponectin receptor protein 1; Progestin and adipoQ receptor family member 1; Progestin and adipoQ receptor family member I

View more View less

Gene Aliases: ACDCR1; ADIPOR1; CGI-45; CGI45; PAQR1; TESBP1A

View more View less

UniProt ID: (Human) Q96A54

View more View less

Entrez Gene ID: (Human) 51094

View more View less

Function(s)

protein kinase binding identical protein binding metal ion binding protein heterodimerization activity adiponectin binding adipokinetic hormone receptor activity

Process(es)

G-protein coupled receptor signaling pathway hormone-mediated signaling pathway negative regulation of epithelial cell migration negative regulation of epithelial to mesenchymal transition regulation of glucose metabolic process regulation of lipid metabolic process fatty acid oxidation negative regulation of cell growth leptin-mediated signaling pathway adiponectin-activated signaling pathway glucose homeostasis negative regulation of JAK-STAT cascade positive regulation of JAK-STAT cascade positive regulation of insulin receptor signaling pathway negative regulation of NIK/NF-kappaB signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Adiponectin receptor 1 Monoclonal Antibody (1F12B3)

Adiponectin receptor 1 Antibody (66619-1-IG) in WB

Product Details

66619-1-IG

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!