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Invitrogen
Aggrecan Recombinant Rabbit Monoclonal Antibody (6L4T2)
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Aggrecan Antibody (MA5-42646) in IHC (P)
Immunohistochemistry analysis of Aggrecan in paraffin-embedded mouse bone marrow. Samples were incubated with Aggrecan Monoclonal antibody (Product # MA5-42646) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
MA5-42646
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6L4T2
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 761-861 of human Aggrecan (P16112).
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911787
Product Specific Information
The target is usually found in the following locations: Secreted, extracellular matrix, extracellular space.
Immunogen sequence: WPPTGAATEE STEGPSATEV PSASEEPSPS EVPFPSEEPS PSEEPFPSVR PFPSVELFPS EEPFPSKEPS PSEEPSASEE PYTPSPPVPS WTELPSSGEE S
Target Information
Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: aggrecan 1; Aggrecan core protein; aggrecan, structural proteoglycan of cartilage; Cartilage-specific proteoglycan core protein; CSPCP
Gene Aliases: Acan; Agc; Agc1; b2b183Clo; cmd; Cspg1
UniProt ID: (Rat) P07897, (Mouse) Q61282
Entrez Gene ID: (Rat) 58968, (Mouse) 11595
cartilage condensation
ossification
chondrocyte development
cell adhesion
central nervous system development
response to radiation
response to mechanical stimulus
response to gravity
response to glucose
multicellular organism aging
response to acidic pH
response to organic cyclic compound
spinal cord development
proteoglycan biosynthetic process
collagen fibril organization
negative regulation of cell migration
response to drug
chondroblast differentiation
cellular response to growth factor stimulus
skeletal system development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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