Invitrogen

Aggrecan Neo Polyclonal Antibody

Aggrecan Neo Antibody in Immunohistochemistry (IHC)

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Aggrecan Neo Antibody (PA1-1746) in IHC

Immunofluorescent analysis of Aggrecan Neo in OA cartilage using a polyclonal antibody (Product # PA1-1746).

Aggrecan Neo Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Aggrecan Neo Antibody in Immunocytochemistry (ICC/IF)

Aggrecan Neo Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Aggrecan Neo Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Aggrecan Neo Antibody in Western Blot (WB)

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Product Details

PA1-1746

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

Assay-dependent

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Pig, Rat

Published species

Bovine, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C G G N(387) I T E G E(392) of human aggrecan.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.83 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2242021

Product Specific Information

PA1-1746 detects human, rat, mouse, bovine, canine, and porcine aggrecan neo G1.

PA1-1746 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~64 kDa protein representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins.

The PA1-1746 immunogen is a synthetic peptide corresponding to residues residues C G G N(387) I T E G E(392) of human aggrecan.

Target Information

Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Synergetic enrichment of aggrecan in nucleus pulposus cells by scAAV6-shRNA-mediated knockdown of aggrecanase-1 and aggrecanase-2.

Experimental biology and medicine (Maywood, N.J.)

Mern DS,Thomé C

Published figure using Aggrecan Neo polyclonal antibody (Product # PA1-1746) in Western Blot

Sat Jul 01 00:00:00 UTC 2023

E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis.

Bioengineering (Basel, Switzerland)

Shi W,Wu J,Pi Y,Yan X,Hu X,Cheng J,Yu H,Shao Z

PA1-1746 was used in Western Blotting to examine differentially expressed genes of BMSCs induced by E7 compared to RGD.

Fri Jun 30 00:00:00 UTC 2023

E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis.

Bioengineering (Basel, Switzerland)

Shi W,Wu J,Pi Y,Yan X,Hu X,Cheng J,Yu H,Shao Z

PA1-1746 was used in Western Blotting to examine differentially expressed genes of BMSCs induced by E7 compared to RGD.

Fri Jun 30 00:00:00 UTC 2023

Maltol ameliorates intervertebral disc degeneration through inhibiting PI3K/AKT/NF-κB pathway and regulating NLRP3 inflammasome-mediated pyroptosis.

Inflammopharmacology

Gong Y,Qiu J,Jiang T,Li Z,Zhang W,Zheng X,He Z,Chen W,Wang Z,Feng X,Wang M,Hong Z

PA1-1746 was used in Western Blotting to suggest that MA slowed IDD development through the PI3K/AKT/NF-κB signaling pathway and NLRP3 inflammasome-mediated pyroptosis, indicating that MA appeared to be a viable medication for IDD treatment.

Wed Feb 01 00:00:00 UTC 2023

Maltol ameliorates intervertebral disc degeneration through inhibiting PI3K/AKT/NF-κB pathway and regulating NLRP3 inflammasome-mediated pyroptosis.

Inflammopharmacology

Gong Y,Qiu J,Jiang T,Li Z,Zhang W,Zheng X,He Z,Chen W,Wang Z,Feng X,Wang M,Hong Z

Wed Feb 01 00:00:00 UTC 2023

Paroxetine Attenuates Chondrocyte Pyroptosis and Inhibits Osteoclast Formation by Inhibiting NF-κB Pathway Activation to Delay Osteoarthritis Progression.

Drug design, development and therapy

Zheng X,Qiu J,Gao N,Jiang T,Li Z,Zhang W,Gong Y,Hong Z,Hong H

PA1-1746 was used in Western Blot to examine whether paroxetine can inhibit pyroptosis and reduce osteoclast formation, thereby delaying the destruction of knee joints.

Wed Aug 23 00:00:00 UTC 2023

Paroxetine Attenuates Chondrocyte Pyroptosis and Inhibits Osteoclast Formation by Inhibiting NF-κB Pathway Activation to Delay Osteoarthritis Progression.

Drug design, development and therapy

Zheng X,Qiu J,Gao N,Jiang T,Li Z,Zhang W,Gong Y,Hong Z,Hong H

PA1-1746 was used in Western Blot to examine whether paroxetine can inhibit pyroptosis and reduce osteoclast formation, thereby delaying the destruction of knee joints.

Wed Aug 23 00:00:00 UTC 2023

Role of Ciliary Protein Intraflagellar Transport Protein 88 in the Regulation of Cartilage Thickness and Osteoarthritis Development in Mice.

Arthritis & rheumatology (Hoboken, N.J.)

Coveney CR,Zhu L,Miotla-Zarebska J,Stott B,Parisi I,Batchelor V,Duarte C,Chang E,McSorley E,Vincent TL,Wann AKT

PA1-1746 was used in Immunohistochemistry to demonstrate that IFT88 is chondroprotective, regulating AC thickness, potentially by thresholding a Hh response to physiological loading that controls cartilage calcification.

Sat Jan 01 00:00:00 UTC 2022

Selumetinib - a potential small molecule inhibitor for osteoarthritis treatment.

Frontiers in pharmacology

Zheng X,Qiu J,Pan W,Gong Y,Zhang W,Jiang T,Chen L,Chen W,Hong Z

Published figure using Aggrecan Neo polyclonal antibody (Product # PA1-1746) in Western Blot

Sat Oct 15 00:00:00 UTC 2022

Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip.

Aging

Xu R,Zhang F,Lu J,Wang K,Pan P,Sun Y,Zhang Y

Published figure using Aggrecan Neo polyclonal antibody (Product # PA1-1746) in Immunocytochemistry

Sun Apr 04 00:00:00 UTC 2021

Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip.

Aging

Xu R,Zhang F,Lu J,Wang K,Pan P,Sun Y,Zhang Y

Published figure using Aggrecan Neo polyclonal antibody (Product # PA1-1746) in Immunocytochemistry

Sun Apr 04 00:00:00 UTC 2021

Platelet-Derived Growth Factor-BB Inhibits Intervertebral Disc Degeneration via Suppressing Pyroptosis and Activating the MAPK Signaling Pathway.

Frontiers in pharmacology

Zhang W,Gong Y,Zheng X,Qiu J,Jiang T,Chen L,Lu F,Wu X,Cheng F,Hong Z

PA1-1746 was used in Western Blot to indicate that PDGF-BB participates in inhibiting the occurrence and development of IDD by inhibiting pyroptosis and regulating the MAPK signaling pathway.

Tue Feb 01 00:00:00 UTC 2022

Vascular dimorphism ensured by regulated proteoglycan dynamics favors rapid umbilical artery closure at birth.

eLife

Nandadasa S,Szafron JM,Pathak V,Murtada SI,Kraft CM,O'Donnell A,Norvik C,Hughes C,Caterson B,Domowicz MS,Schwartz NB,Tran-Lundmark K,Veigl M,Sedwick D,Philipson EH,Humphrey JD,Apte SS

PA1-1746 was used in Immunocytochemistry to analyse of umbilical cords from humans and other mammals identified differential arterial-venous proteoglycan dynamics as a determinant of these contrasting vascular responses.

Thu Sep 10 00:00:00 UTC 2020

Vascular dimorphism ensured by regulated proteoglycan dynamics favors rapid umbilical artery closure at birth.

eLife

Nandadasa S,Szafron JM,Pathak V,Murtada SI,Kraft CM,O'Donnell A,Norvik C,Hughes C,Caterson B,Domowicz MS,Schwartz NB,Tran-Lundmark K,Veigl M,Sedwick D,Philipson EH,Humphrey JD,Apte SS

Thu Sep 10 00:00:00 UTC 2020

An in vitro and in vivo comparison of cartilage growth in chondrocyte-laden matrix metalloproteinase-sensitive poly(ethylene glycol) hydrogels with localized transforming growth factor β3.

Acta biomaterialia

Schneider MC,Chu S,Randolph MA,Bryant SJ

Published figure using Aggrecan Neo polyclonal antibody (Product # PA1-1746) in Immunocytochemistry

Mon Jul 15 00:00:00 UTC 2019

Glycoproteomic Analysis of the Aortic Extracellular Matrix in Marfan Patients.

Arteriosclerosis, thrombosis, and vascular biology

Yin 殷晓科 X,Wanga S,Fellows AL,Barallobre-Barreiro J,Lu R,Davaapil H,Franken R,Fava M,Baig F,Skroblin P,Xing Q,Koolbergen DR,Groenink M,Zwinderman AH,Balm R,de Vries CJM,Mulder BJM,Viner R,Jahangiri M,Reinhardt DP,Sinha S,de Waard V,Mayr M

PA1-1746 was used in Western Blotting to characterise the glycoproteome of the aortic extracellular matrix from patients with Marfan syndrome and relate it to aortopathy.

Sun Sep 01 00:00:00 UTC 2019

Extracellular Matrix Proteomics Reveals Interplay of Aggrecan and Aggrecanases in Vascular Remodeling of Stented Coronary Arteries.

Circulation

Suna G,Wojakowski W,Lynch M,Barallobre-Barreiro J,Yin X,Mayr U,Baig F,Lu R,Fava M,Hayward R,Molenaar C,White SJ,Roleder T,Milewski KP,Gasior P,Buszman PP,Buszman P,Jahangiri M,Shanahan CM,Hill J,Mayr M

Published figure using Aggrecan Neo polyclonal antibody (Product # PA1-1746) in Immunohistochemistry (Paraffin)

Tue Jan 09 00:00:00 UTC 2018

Extracellular Matrix Proteomics Reveals Interplay of Aggrecan and Aggrecanases in Vascular Remodeling of Stented Coronary Arteries.

Circulation

Published figure using Aggrecan Neo polyclonal antibody (Product # PA1-1746) in Immunohistochemistry (Paraffin)

Tue Jan 09 00:00:00 UTC 2018

Extracellular Matrix Proteomics Reveals Interplay of Aggrecan and Aggrecanases in Vascular Remodeling of Stented Coronary Arteries.

Circulation

Published figure using Aggrecan Neo polyclonal antibody (Product # PA1-1746) in Immunohistochemistry (Paraffin)

Tue Jan 09 00:00:00 UTC 2018

Suramin increases cartilage proteoglycan accumulation in vitro and protects against joint damage triggered by papain injection in mouse knees in vivo.

RMD open

Guns LA,Monteagudo S,Kvasnytsia M,Kerckhofs G,Vandooren J,Opdenakker G,Lories RJ,Cailotto F

Published figure using Aggrecan Neo polyclonal antibody (Product # PA1-1746) in Immunohistochemistry

Mon Mar 14 00:00:00 UTC 2022

Brief Report: JNK-2 Controls Aggrecan Degradation in Murine Articular Cartilage and the Development of Experimental Osteoarthritis.

Arthritis & rheumatology (Hoboken, N.J.)

Ismail HM,Miotla-Zarebska J,Troeberg L,Tang X,Stott B,Yamamoto K,Nagase H,Fosang AJ,Vincent TL,Saklatvala J

PA1-1746 was used in immunohistochemistry - paraffin section to analyze development of experimental osteoarthritis and aggrecan degradation in murine articular cartilage by JNK-2

Sun May 01 00:00:00 UTC 2016

Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption.

Scientific reports

Mével E,Merceron C,Vinatier C,Krisa S,Richard T,Masson M,Lesoeur J,Hivernaud V,Gauthier O,Abadie J,Nourissat G,Houard X,Wittrant Y,Urban N,Beck L,Guicheux J

PA1-1746 was used in immunohistochemistry - paraffin section determine the effects of hydroxytyrosol and procyanidin treatment on osteoarthritis using rabbits

Mon Sep 19 00:00:00 UTC 2016

Transient anabolic effects accompany epidermal growth factor receptor signal activation in articular cartilage in vivo.

Arthritis research & therapy

Shepard JB,Jeong JW,Maihle NJ,O'Brien S,Dealy CN

PA1-1746 was used in immunohistochemistry to study EGFR-mediated anabolic signaling in articular cartilage using Mig6 conditional knockout mice

Fri Nov 14 00:00:00 UTC 2014

Interleukin-1alpha treatment of meniscal explants stimulates the production and release of aggrecanase-generated, GAG-substituted aggrecan products and also the release of pre-formed, aggrecanase-generated G1 and m-calpain-generated G1-G2.

Cell and tissue research

Lemke AK,Sandy JD,Voigt H,Dreier R,Lee JH,Grodzinsky AJ,Mentlein R,Fay J,Schünke M,Kurz B

PA1-1746 was used in Immunohistochemistry-immunofluorescence to conclude that aggrecanases are responsible for IL-1-stimulated GAG release from meniscal explants, and that IL-1 also stimulates release of G1-bearing products, by a process possibly involving hyaluronan fragmentation.

Thu Apr 01 00:00:00 UTC 2010

Transcription factor Nfat1 deficiency causes osteoarthritis through dysfunction of adult articular chondrocytes.

The Journal of pathology

Wang J,Gardner BM,Lu Q,Rodova M,Woodbury BG,Yost JG,Roby KF,Pinson DM,Tawfik O,Anderson HC

PA1-1746 was used in immunohistochemistry to study the role of transcription factor NFAT1 in osteoarthritis and its mechanism

Thu Oct 01 00:00:00 UTC 2009

Analysis of aggrecan in human knee cartilage and synovial fluid indicates that aggrecanase (ADAMTS) activity is responsible for the catabolic turnover and loss of whole aggrecan whereas other protease activity is required for C-terminal processing in vivo.

The Biochemical journal

Sandy JD,Verscharen C

PA1-1746 was used in western blot to investigate the effect of ADAMTS activities and MMP-like activities on cartilage matrix

Sat Sep 15 00:00:00 UTC 2001

The intermediates of aggrecanase-dependent cleavage of aggrecan in rat chondrosarcoma cells treated with interleukin-1.

The Biochemical journal

Sandy JD,Thompson V,Doege K,Verscharen C

PA1-1746 was used in western blot to investigate the degradation products of aggrecan by aggrecanase in interleukin 1-treated rat chondrosarcoma cells

Sun Oct 01 00:00:00 UTC 2000

Bioinformatics

Protein Aliases: aggrecan 1; aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); Aggrecan core protein; aggrecan epidermal growth factor-like domain-1; aggrecan, structural proteoglycan of cartilage; cartilage aggregating proteoglycan; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; large aggregating proteoglycan; large aggregating proteoglycan, antigen

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Gene Aliases: ACAN; Agc; AGC1; AGCAN; b2b183Clo; BOS_20116; cmd; CSPCP; CSPG1; CSPGCP; MSK16; SEDK

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UniProt ID: (Bovine) P13608, (Human) P16112, (Rat) P07897, (Mouse) Q61282

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Entrez Gene ID: (Dog) 403828, (Pig) 397255, (Bovine) 280985, (Human) 176, (Rat) 58968, (Mouse) 11595

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Function(s)

calcium ion binding hyaluronic acid binding carbohydrate binding protein binding extracellular matrix structural constituent metal ion binding

Process(es)

skeletal system development cell adhesion central nervous system development cartilage condensation ossification chondrocyte development response to radiation response to mechanical stimulus response to gravity response to glucose multicellular organism aging response to acidic pH response to organic cyclic compound spinal cord development proteoglycan biosynthetic process collagen fibril organization negative regulation of cell migration response to drug chondroblast differentiation cellular response to growth factor stimulus proteolysis keratan sulfate biosynthetic process extracellular matrix disassembly extracellular matrix organization keratan sulfate catabolic process

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Aggrecan Neo Polyclonal Antibody

Aggrecan Neo Antibody (PA1-1746) in IHC

Product Details

PA1-1746

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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