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Ago1/2/3 Antibody (39937) in IP
Ago1/2/3 antibody (mAb) (Clone 2A8) tested by immunoprecipitation. The Active Motif miRNA IP Kit was used on samples of HT1080 cells that had been transfected with a miR-122 or a non-targeting miRNA control for 8 hours. Following IP using the Ago1/2/3 antibody or negative control IgG, qRT-PCR was performed on the samples using primers for G6PC3 and SLC1A5, which are known targets of miR-122, and for GAPDH, a common housekeeping gene that is not known to be targeted by miR-122.
Product Details
39937
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
2A8
Immunogen
This Ago1/2/3 antibody was raised against a recombinant protein corresponding to amino acids 47-859 of human Ago2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
70mM tris, pH 8, with 30% glycerol, 105mM NaCl, 31mM glycine, 0.07mM EDTA
Contains
0.035% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2793399
Target Information
The Argonaute family of proteins play roles in RNA interference and in short-interfering-RNA-mediated gene silencing. Small interfering RNAs (siRNAs) have gained much attention for their powerful ability to suppress gene expression. Introduction of double-stranded RNA (dsRNA), that are homologues in sequence to a gene, has proven to suppress that gene's expression through a process known as RNA interference (RNAi); this process post-transcriptionally silences a gene through mRNA inhibition or degradation. Both biochemical and genetic studies have led to the identification of two, conserved gene families that are universal components of the interference process. These are the Dicer family, which is comprised of members of the Ribonuclease III family of enzymes, and the Argonaute gene family. Argonaute proteins addition to their role in RNAi process, may also influence development, such as, stem cell fate determination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: argonaute 1; argonaute 2; argonaute 3; Argonaute RISC catalytic component 1; Argonaute RISC catalytic component 2; Argonaute RISC catalytic component 3; Argonaute1; Argonaute2; Argonaute3; CTA-204B4.6; eIF-2C 1; eIF-2C 2; eIF-2C 3; eIF2C 1; eIF2C 2; Eukaryotic translation initiation factor 2C 1; Eukaryotic translation initiation factor 2C 2; Eukaryotic translation initiation factor 2C 3; eukaryotic translation initiation factor 2C, 1; eukaryotic translation initiation factor 2C, 2; eukaryotic translation initiation factor 2C, 3; Golgi Endoplasmic Reticulum protein 95 kDa; hAgo2; hAgo3; PAZ Piwi domain protein; PPD; Protein argonaute-1; Protein argonaute-2; Protein argonaute-3; Protein slicer; Putative RNA-binding protein Q99
Gene Aliases: AGO1; AGO2; AGO3; EIF2C; EIF2C1; EIF2C2; EIF2C3; GERP95; hAgo1; Q10; Q99
UniProt ID: (Human) Q9UL18, (Human) Q9UKV8, (Human) Q9H9G7
Entrez Gene ID: (Human) 26523, (Human) 27161, (Human) 192669
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core binding
core promoter binding
RNA binding
double-stranded RNA binding
single-stranded RNA binding
endoribonuclease activity
protein binding
miRNA binding
poly(A) RNA binding
RNA 7-methylguanosine cap binding
mRNA binding
translation initiation factor activity
protein C-terminus binding
siRNA binding
metal ion binding
endoribonuclease activity, cleaving siRNA-paired mRNA
endoribonuclease activity, cleaving miRNA-paired mRNA
nuclear-transcribed mRNA catabolic process
transcription, DNA-templated
Wnt signaling pathway, calcium modulating pathway
RNA secondary structure unwinding
miRNA metabolic process
gene silencing by RNA
pre-miRNA processing
posttranscriptional gene silencing by RNA
production of miRNAs involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA cleavage involved in gene silencing by miRNA
miRNA loading onto RISC involved in gene silencing by miRNA
positive regulation of transcription from RNA polymerase II promoter
phosphatidylinositol-mediated signaling
mRNA cleavage involved in gene silencing by siRNA
mRNA catabolic process
regulation of stem cell proliferation
translation
translational initiation
post-embryonic development
siRNA loading onto RISC involved in RNA interference
negative regulation of translational initiation
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
RNA phosphodiester bond hydrolysis, endonucleolytic
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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