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Invitrogen
Agrin Polyclonal Antibody
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FIGURE: 1 / 4
Agrin Antibody (PA5-112353) in ICC/IF
Immunocytochemical analysis of Agrin in Hela cells using a Agrin polyclonal antibody (Product # PA5-112353) at a dilution of 1:133. The cells were treated with 30mM sodium butyrate for 4 hours and counter stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the primary antibody overnight at 4°C. The secondary antibody was an Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).
Product Details
PA5-112353
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Agrin protein (968-1130AA)
Conjugate
Form
Liquid
Concentration
4.72 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2867089
Target Information
Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designated "y" and "z" display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly. Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention. Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation. Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Agrin; agrin proteoglycan; RP23-139J18.2
Gene Aliases: AGRIN; AGRN; CMS8; CMSPPD
UniProt ID: (Human) O00468
Entrez Gene ID: (Human) 375790
retinoid metabolic process
glycosaminoglycan biosynthetic process
glycosaminoglycan catabolic process
cytoskeleton organization
signal transduction
G-protein coupled acetylcholine receptor signaling pathway
extracellular matrix organization
glycosaminoglycan metabolic process
receptor clustering
positive regulation of GTPase activity
clustering of voltage-gated sodium channels
positive regulation of synaptic growth at neuromuscular junction
positive regulation of transcription from RNA polymerase II promoter
synapse organization
positive regulation of filopodium assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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