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Aha1 Antibody (200-501-974) in IHC (P)
Rocklands anti-AHA1 monoclonal antibody was used at a 5-10 µg/mL to detect AHA1 in the seminiferous tubule of human testis (40X) showing moderate staining. Leydig cells showed faint to moderate staining. Expression of AHA1 is reported in many epithelial and lymphatic tissues, with cytoplasmic localization. This antibody showed moderate cytoplasmic staining of a variety of epithelial tissues and lymphoid org... View More
Product Details
200-501-974
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chimpanzee,
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
25F2.D9
Immunogen
This Protein G purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse AHA1 protein followed by hybridoma development.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
It is directed against human Aha1 protein. A BLAST analysis was used to suggest cross-reactivity with Aha1 protein from mouse, human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
Acts as a co-chaperone of HSP90AA1 (PubMed:29127155). Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity (PubMed:29127155). Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360). Competes with the inhibitory co-chaperone TSC1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:29127155). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activator of 90 kDa heat shock protein ATPase homolog 1; Aha 1; AHA1; AHA1, activator of heat shock 90kDa protein ATPase homolog 1; AHA1, activator of heat shock protein ATPase homolog 1; AHSA 1; p38; SHSA1
Gene Aliases: AHA1; AHSA1; BC023857; C14orf3; hAha1; HSPC322; p38
UniProt ID: (Human) O95433, (Mouse) Q8BK64
Entrez Gene ID: (Human) 10598, (Mouse) 217737
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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