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Rockland
Aldehyde Dehydrogenase Polyclonal Antibody, HRP
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FIGURE: 1 / 1
Aldehyde Dehydrogenase Antibody (200-4344-0100) in WB
Western Blot of Rabbit anti-Aldehyde Dehydrogenase (yeast) Antibody Peroxidase Conjugated. Lane 1: Aldehyde Dehydrogenase (yeast). Load: 50 ng per lane. Primary antibody: Rabbit anti-Aldehyde Dehydrogenase (yeast) Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Aldehyde Dehydrogenase (yeast).
Product Details
200-4344-0100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Aldehyde Dehydrogenase [Yeast]
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA
Contains
0.01% gentamicin sulfate
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Aldehyde dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast]. Cross reactivity against Aldehyde Dehydrogenase from other sources is unknown.
Target Information
Cytoplasmic aldehyde dehydrogenase is involved in ethanol oxidation. Aldehyde dehydrogenase is required for pantothenic acid production through the conversion of 3-aminopropanal to beta-alanine, an intermediate in pantothenic acid (vitamin B5) and coenzyme A (CoA) biosynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aldehyde dehydrogenase (NAD(+)) ALD2; Aldehyde dehydrogenase [NAD(P)+] 1
Gene Aliases: ALD2; ALD5; YM8520.19C; YMR170C
UniProt ID: (Baker's yeast) P47771
Entrez Gene ID: (Baker's yeast) 855206
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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