Invitrogen

Aldolase A/B/C Recombinant Rabbit Monoclonal Antibody (PSH01-81)

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-56379

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2000-1:5000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:20,000
-

Immunohistochemistry (Frozen) (IHC (F))

1:1,000
-

Immunocytochemistry (ICC/IF)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PSH01-81

Immunogen

Synthetic peptide within human aldolase c aa 50-100.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Positive Control: U-87 MG cell lysate, RAW264.7 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, mouse pancreas tissue lysate, rat brain tissue lysate, rat pancreas tissue lysate, U-87 MG, mouse brain tissue, rat brain tissue.

Tissue Specificity: Group enriched (brain, retina).

Subcellular Location: cytoskeleton, cytosol, extracellular exosome, extracellular region.

Sequence Similarities: 100% Mouse/Rat.

Predicted band size: 110 kDa.

Target Information

Aldolases are important glycolytic cytosolic enzymes which catalyse the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate. There are three aldolase isozymes coded by three distinct genes in mammals, namely aldolases A, B, and C. Aldolase A is heavily expressed in muscle enzyme and aldolase B is heavily expressed in liver and is regarded as a liver specific enzyme. In the adult, aldolase C is the brain-specific isozyme expressed in astrocytes and a few classes of neurons, notably Purkinje cells. Appropriate antibodies to aldolase C are therefore useful to identify astrocytes in cell culture and sectioned material, and the enzyme may be over expressed in some forms of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ALDA; ALDB; ALDC; Aldolase 1; aldolase 1, A isoform; Aldolase 2; aldolase 2, B isoform; Aldolase 3; aldolase A, fructose-bisphosphate; Aldolase B fructose-biphosphate; Aldolase B, fructose-biphosphate; aldolase B, fructose-bisphosphatase; aldolase B, fructose-bisphosphate; aldolase C, fructose-biphosphate; aldolase C, fructose-bisphosphate; Brain-type aldolase; epididymis secretory sperm binding protein Li 87p; fructoaldolase C; fructose-1,6-biphosphate triosephosphate lyase; fructose-1,6-bisphosphate triosephosphate-lyase; Fructose-bisphosphate aldolase A; Fructose-bisphosphate aldolase B; Fructose-bisphosphate aldolase C; Liver-type aldolase; Lung cancer antigen NY-LU-1; Muscle-type aldolase; Scrapie-responsive protein 2; Zebrin II

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Gene Aliases: AI847350; ALDA; ALDB; ALDC; ALDCAA; Aldo-1; Aldo-2; Aldo1; ALDO2; Aldo3; ALDOA; ALDOB; ALDOC; AU040929; BC016435; F16dip7; GSD12; HEL-S-87p; LIV10; RATALDCAA; RNALDOG5; Scrg2

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UniProt ID: (Human) P04075, (Human) P05062, (Human) P09972, (Mouse) P05064, (Rat) P05065, (Mouse) Q91Y97, (Mouse) P05063, (Rat) P09117

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Entrez Gene ID: (Human) 226, (Human) 229, (Human) 230, (Mouse) 11674, (Rat) 24189, (Mouse) 230163, (Rat) 24190, (Mouse) 11676, (Rat) 24191

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Function(s)
actin binding fructose-bisphosphate aldolase activity protein binding cytoskeletal protein binding tubulin binding identical protein binding poly(A) RNA binding fructose binding cadherin binding involved in cell-cell adhesion phosphatidylcholine binding ATPase binding fructose-1-phosphate aldolase activity protease binding molecular_function catalytic activity lyase activity aldolase
Process(es)
platelet degranulation fructose metabolic process gluconeogenesis glycolytic process ATP biosynthetic process striated muscle contraction actin filament organization regulation of cell shape fructose 1,6-bisphosphate metabolic process muscle cell cellular homeostasis protein homotetramerization canonical glycolysis cell-cell adhesion liver development NADH oxidation response to fructose response to zinc ion cellular response to extracellular stimulus positive regulation of ATPase activity cellular response to insulin stimulus response to drug response to starvation response to amino acid response to copper ion response to glucocorticoid response to cAMP fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate vacuolar proton-transporting V-type ATPase complex assembly response to interleukin-6 epithelial cell differentiation response to hypoxia response to heat response to lipopolysaccharide response to nicotine response to estrogen response to peptide hormone response to carbohydrate response to organic cyclic compound apoptotic process aging response to organonitrogen compound organ regeneration protein heterotetramerization
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