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Rockland
Aldolase Polyclonal Antibody
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FIGURE: 1 / 4
Aldolase Antibody (200-1141-0100) in WB
Western Blot of Goat anti-Aldolase Antibody. Lane 1: Hela lysate (p/n W09-000-364). Lane 2: HEK293 lysate (p/n W09-000-365). Lane 3: Jurkat lysate (p/n W09-001-370). Load: 25 µg per lane. Primary antibody: Goat anti-Aldolase Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP Dk-a-Gt IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 39 kDa, 41 kDa for Aldolase.
Product Details
200-1141-0100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rabbit
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Aldolase [Rabbit Muscle]
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-ALDOLASE was prepared from monospecific antiserum by a delipidation, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, purified and partially purified Aldolase [Rabbit Muscle].
Target Information
Aldolase encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aldolase A, fructose-bisphosphate; epididymis secretory sperm binding protein Li 87p; fructose-1,6-bisphosphate triosephosphate-lyase; Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase
Gene Aliases: ALDA; ALDOA; GSD12; HEL-S-87p
UniProt ID: (Rabbit) P00883, (Human) P04075
Entrez Gene ID: (Rabbit) 100009055, (Human) 226
platelet degranulation
fructose metabolic process
gluconeogenesis
glycolytic process
ATP biosynthetic process
striated muscle contraction
actin filament organization
regulation of cell shape
fructose 1,6-bisphosphate metabolic process
muscle cell cellular homeostasis
protein homotetramerization
canonical glycolysis
cell-cell adhesion
Performance Guarantee
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