Invitrogen

Alix Monoclonal Antibody (3A9)

Alix Antibody in Immunocytochemistry (ICC/IF)

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Alix Antibody (MA1-83977) in ICC/IF

Immunofluorescence analysis of Alix was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Alix Monoclonal Antibody (3A9) (Product # MA1-83977) at 5µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1... View More

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Alix Antibody — Antibody specificity was demonstrated by siRNA mediated KD of target protein. HeLa cells were transfected with Alix siRNA and loss of signal was observed in Western Blot using Alix Monoclonal Antibody (3A9) (Product # MA1-83977). {KD}

Product Details

MA1-83977

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunocytochemistry (ICC/IF)

5 µg/mL

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Dot blot (DB)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat, Xenopus

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3A9

Immunogen

Alix fusion protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2162469

Product Specific Information

This product does not require protein digestion pre-treatment of paraffin sections. A ~100kD band is detected by Western blot.

Mouse anti ALIX antibody, clone 3A9 recognizes the apoptosis-linked gene-2 interacting protein X (ALIX), also known as Programmed cell death 6-interacting protein or Hp95.

Target Information

Apoptotic linked-gene-product 2 (ALG-2) interacting protein X (ALIX) is a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cardiac progenitor cell-derived extracellular vesicles promote angiogenesis through both associated- and co-isolated proteins.

Communications biology

Roefs MT,Bauzá-Martinez J,van de Wakker SI,Qin J,Olijve WT,Tuinte R,Rozeboom M,Snijders Blok C,Mol EA,Wu W,Vader P,Sluijter JPG

MA1-83977 was used in Western Blotting to discovered that the ability of CPC-EVs to induce in vitro angiogenesis and to stimulate pro-survival pathways was lost upon EV donor cell exposure to calcium ionophore.

Tue Aug 01 00:00:00 UTC 2023

Cardiac progenitor cell-derived extracellular vesicles promote angiogenesis through both associated- and co-isolated proteins.

Communications biology

Roefs MT,Bauzá-Martinez J,van de Wakker SI,Qin J,Olijve WT,Tuinte R,Rozeboom M,Snijders Blok C,Mol EA,Wu W,Vader P,Sluijter JPG

Tue Aug 01 00:00:00 UTC 2023

In vitro reconstitution of calcium-dependent recruitment of the human ESCRT machinery in lysosomal membrane repair.

Proceedings of the National Academy of Sciences of the United States of America

Shukla S,Larsen KP,Ou C,Rose K,Hurley JH

Published figure using Alix monoclonal antibody (Product # MA1-83977) in Immunocytochemistry

Tue Aug 30 00:00:00 UTC 2022

Muscle cells of sporadic amyotrophic lateral sclerosis patients secrete neurotoxic vesicles.

Journal of cachexia, sarcopenia and muscle

Le Gall L,Duddy WJ,Martinat C,Mariot V,Connolly O,Milla V,Anakor E,Ouandaogo ZG,Millecamps S,Lainé J,Vijayakumar UG,Knoblach S,Raoul C,Lucas O,Loeffler JP,Bede P,Behin A,Blasco H,Bruneteau G,Del Mar Amador M,Devos D,Henriques A,Hesters A,Lacomblez L,Laforet P,Langlet T,Leblanc P,Le Forestier N,Maisonobe T,Meininger V,Robelin L,Salachas F,Stojkovic T,Querin G,Dumonceaux J,Butler Browne G,González De Aguilar JL,Duguez S,Pradat PF

MA1-83977 was used in Western Blot to test a hypothesis that muscle vesicles may be involved in ALS pathology.

Fri Apr 01 00:00:00 UTC 2022

Plasma exosomal miR-122 regulates the efficacy of metformin via AMPK in type 2 diabetes and hepatocellular carcinoma.

Heliyon

Peng H,Hou M,Wu Z,Wang J,Zhou M,Zhuang X,Xing J,Tao Q,Huang L,Zhou F,Zhang S,Feng Q,Hou Y,Yu Q

MA1-83977 was used in Western Blot to reveal a key regulatory role of plasma exosomal miR-122 on the molecular mechanism of metformin.

Tue Nov 01 00:00:00 UTC 2022

Protein content of blood-derived extracellular vesicles: An approach to the pathophysiology of cerebral hemorrhage.

Frontiers in cellular neuroscience

Laso-García F,Piniella D,Gómez-de Frutos MC,Casado-Fernández L,Pérez-Mato M,Alonso-López E,Otero-Ortega L,Bravo SB,Chantada-Vázquez MDP,Trilla-Fuertes L,Fresno-Vara JÁ,Fuentes B,Díez-Tejedor E,Gutiérrez-Fernández M,Alonso De Leciñana M

MA1-83977 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to describe the number and protein content of blood-derived EVs following an intracerebral hemorrhage (ICH),.

Tue Feb 14 00:00:00 UTC 2023

Protein content of blood-derived extracellular vesicles: An approach to the pathophysiology of cerebral hemorrhage.

Frontiers in cellular neuroscience

Tue Feb 14 00:00:00 UTC 2023

Neddylation-mediated degradation of hnRNPA2B1 contributes to hypertriglyceridemia pancreatitis.

Cell death & disease

Chen W,Wang Y,Xia W,Zhang J,Zhao Y

MA1-83977 was used in Immunohistochemistry to indicate that pharmacological inhibitors that target neddylation may provide therapeutic benefits to HTGP patients.

Tue Oct 11 00:00:00 UTC 2022

Cerebral endothelial cell-derived small extracellular vesicles enhance neurovascular function and neurological recovery in rat acute ischemic stroke models of mechanical thrombectomy and embolic stroke treatment with tPA.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Li C,Wang C,Zhang Y,Alsrouji OK,Chebl AB,Ding G,Jiang Q,Mayer SA,Lu M,Kole MK,Marin HL,Zhang L,Chopp M,Zhang ZG

MA1-83977 was used in Western Blotting to conclude that CEC-sEVs have a therapeutic effect on acute ischemic stroke by reducing neurovascular damage.

Sun Aug 01 00:00:00 UTC 2021

Porphyromonas gingivalis Provokes Exosome Secretion and Paracrine Immune Senescence in Bystander Dendritic Cells.

Frontiers in cellular and infection microbiology

Elsayed R,Elashiry M,Liu Y,El-Awady A,Hamrick M,Cutler CW

MA1-83977 was used in Western Blotting to examine whether immune cells typical of those at the oral mucosa-microbe interface, are vulnerable to cellular senescence (CS) and the role of dysbiotic oral pathogen Porphyromonas gingivalis.

Mon Jul 05 00:00:00 UTC 2021

Porphyromonas gingivalis Provokes Exosome Secretion and Paracrine Immune Senescence in Bystander Dendritic Cells.

Frontiers in cellular and infection microbiology

Elsayed R,Elashiry M,Liu Y,El-Awady A,Hamrick M,Cutler CW

Mon Jul 05 00:00:00 UTC 2021

Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV-CATCHER): A customizable purification assay designed for small-RNA biomarker identification and evaluation of circulating small-EVs.

Journal of extracellular vesicles

Mitchell MI,Ben-Dov IZ,Liu C,Ye K,Chow K,Kramer Y,Gangadharan A,Park S,Fitzgerald S,Ramnauth A,Perlin DS,Donato M,Bhoy E,Manouchehri Doulabi E,Poulos M,Kamali-Moghaddam M,Loudig O

Published figure using Alix monoclonal antibody (Product # MA1-83977) in Western Blot

Tue Jun 01 00:00:00 UTC 2021

Extracellular Vesicles Secreted by Mesenchymal Stromal Cells Exert Opposite Effects to Their Cells of Origin in Murine Sodium Dextran Sulfate-Induced Colitis.

Frontiers in immunology

Tolomeo AM,Castagliuolo I,Piccoli M,Grassi M,Magarotto F,De Lazzari G,Malvicini R,Caicci F,Franzin C,Scarpa M,Macchi V,De Caro R,Angriman I,Viola A,Porzionato A,Pozzobon M,Muraca M

MA1-83977 was used in Western Blotting to compare the effects of MSCs and of MSC-EV administration in mice with colitis induced by dextran sulfate sodium.

Wed Sep 15 00:00:00 UTC 2021

Extracellular Vesicles Secreted by Mesenchymal Stromal Cells Exert Opposite Effects to Their Cells of Origin in Murine Sodium Dextran Sulfate-Induced Colitis.

Frontiers in immunology

Tolomeo AM,Castagliuolo I,Piccoli M,Grassi M,Magarotto F,De Lazzari G,Malvicini R,Caicci F,Franzin C,Scarpa M,Macchi V,De Caro R,Angriman I,Viola A,Porzionato A,Pozzobon M,Muraca M

MA1-83977 was used in Western Blotting to compare the effects of MSCs and of MSC-EV administration in mice with colitis induced by dextran sulfate sodium.

Wed Sep 15 00:00:00 UTC 2021

Antisense oligonucleotide activity in tumour cells is influenced by intracellular LBPA distribution and extracellular vesicle recycling.

Communications biology

Kapustin AN,Davey P,Longmire D,Matthews C,Linnane E,Rustogi N,Stavrou M,Devine PWA,Bond NJ,Hanson L,Sonzini S,Revenko A,MacLeod AR,Ross S,Chiarparin E,Puri S

MA1-83977 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to compare intracellular traffic of anti KRAS antisense oligonucleotide (AZD4785) in tumour cell lines PC9 and LK2, with good and poor productive uptake, respectively.

Mon Nov 01 00:00:00 UTC 2021

Antisense oligonucleotide activity in tumour cells is influenced by intracellular LBPA distribution and extracellular vesicle recycling.

Communications biology

Kapustin AN,Davey P,Longmire D,Matthews C,Linnane E,Rustogi N,Stavrou M,Devine PWA,Bond NJ,Hanson L,Sonzini S,Revenko A,MacLeod AR,Ross S,Chiarparin E,Puri S

Mon Nov 01 00:00:00 UTC 2021

Oral cancer cell‑derived exosomes modulate natural killer cell activity by regulating the receptors on these cells.

International journal of molecular medicine

Zhu X,Qin X,Wang X,Wang Y,Cao W,Zhang J,Chen W

Published figure using Alix monoclonal antibody (Product # MA1-83977) in Western Blot

Tue Dec 01 00:00:00 UTC 2020

Restoration of lysosomal function after damage is accompanied by recycling of lysosomal membrane proteins.

Cell death & disease

Eriksson I,Wäster P,Öllinger K

MA1-83977 was used in Immunocytochemistry-immunoflourescence to investigate the premises for lysosomal membrane repair after lysosomal membrane permeabilization (LMP).

Thu May 14 00:00:00 UTC 2020

Restoration of lysosomal function after damage is accompanied by recycling of lysosomal membrane proteins.

Cell death & disease

Eriksson I,Wäster P,Öllinger K

MA1-83977 was used in Immunocytochemistry-immunoflourescence to investigate the premises for lysosomal membrane repair after lysosomal membrane permeabilization (LMP).

Thu May 14 00:00:00 UTC 2020

A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA.

Nature communications

de Jong OG,Murphy DE,Mäger I,Willms E,Garcia-Guerra A,Gitz-Francois JJ,Lefferts J,Gupta D,Steenbeek SC,van Rheenen J,El Andaloussi S,Schiffelers RM,Wood MJA,Vader P

MA1-83977 was used in Western Blot to allow the elucidation of regulatory mechanisms in EV-mediated RNA transfer at the level of EV biogenesis, endocytosis, intracellular trafficking, and RNA delivery.

Fri Feb 28 00:00:00 UTC 2020

Assessment of methods for serum extracellular vesicle small RNA sequencing to support biomarker development.

Journal of extracellular vesicles

Srinivasan S,Duval MX,Kaimal V,Cuff C,Clarke SH

Published figure using Alix monoclonal antibody (Product # MA1-83977) in Dot blot

Thu Oct 19 00:00:00 UTC 2023

CCL2 mobilizes ALIX to facilitate Gag-p6 mediated HIV-1 virion release.

eLife

Ajasin DO,Rao VR,Wu X,Ramasamy S,Pujato M,Ruiz AP,Fiser A,Bresnick AR,Kalpana GV,Prasad VR

MA1-83977 was used in Western Blotting to show that CCL2-mediated increase in virus production requires Gag late motif LYPX present in HIV-1B, but absent in HIV-1C, and ALIX protein that recruits ESCRT III complex.

Fri Jun 07 00:00:00 UTC 2019

CCL2 mobilizes ALIX to facilitate Gag-p6 mediated HIV-1 virion release.

eLife

Ajasin DO,Rao VR,Wu X,Ramasamy S,Pujato M,Ruiz AP,Fiser A,Bresnick AR,Kalpana GV,Prasad VR

Fri Jun 07 00:00:00 UTC 2019

Alpha-synuclein is strategically positioned for afferent modulation of midbrain dopamine neurons and is essential for cocaine preference.

Communications biology

Trubetckaia O,Lane AE,Qian L,Zhou P,Lane DA

Published figure using Alix monoclonal antibody (Product # MA1-83977) in Western Blot

Mon May 18 00:00:00 UTC 2020

Exosomes From Astrocyte Processes: Signaling to Neurons.

Frontiers in pharmacology

Venturini A,Passalacqua M,Pelassa S,Pastorino F,Tedesco M,Cortese K,Gagliani MC,Leo G,Maura G,Guidolin D,Agnati LF,Marcoli M,Cervetto C

Published figure using Alix monoclonal antibody (Product # MA1-83977) in Western Blot

Thu Oct 01 00:00:00 UTC 2020

AQP1-Containing Exosomes in Peritoneal Dialysis Effluent As Biomarker of Dialysis Efficiency.

Cells

Corciulo S,Nicoletti MC,Mastrofrancesco L,Milano S,Mastrodonato M,Carmosino M,Gerbino A,Corciulo R,Russo R,Svelto M,Gesualdo L,Procino G

MA1-83977 was used in Western Blotting to report on the functional role of mesothelial AQP1 during PD and suggests that it may represent a potential non-invasive biomarker of peritoneal barrier integrity, with predictive potential of ultrafiltration failure in PD patients.

Tue Apr 09 00:00:00 UTC 2019

Acidic microenvironment plays a key role in human melanoma progression through a sustained exosome mediated transfer of clinically relevant metastatic molecules.

Journal of experimental & clinical cancer research : CR

Boussadia Z,Lamberti J,Mattei F,Pizzi E,Puglisi R,Zanetti C,Pasquini L,Fratini F,Fantozzi L,Felicetti F,Fecchi K,Raggi C,Sanchez M,D'Atri S,Carè A,Sargiacomo M,Parolini I

Published figure using Alix monoclonal antibody (Product # MA1-83977) in Western Blot

Fri Oct 05 00:00:00 UTC 2018

Microenvironmental pH and Exosome Levels Interplay in Human Cancer Cell Lines of Different Histotypes.

Cancers

Logozzi M,Mizzoni D,Angelini DF,Di Raimo R,Falchi M,Battistini L,Fais S

MA1-83977 was used in Western Blotting to show an increase in exosome release was observed at pH 6.5, indicating tumor acidity and exosome levels represent common cancer phenotypes.

Fri Oct 05 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: ALG-2 interacting protein 1; ALG-2 interacting protein X; ALG-2-interacting protein 1; ALG-2-interacting protein X; Alg2-interacting protein 1; Alg2-interacting protein X; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; E2F1-inducible protein; Eig2; Hp95; MGC17003; PDCD6-interacting protein; Programmed cell death 6-interacting protein; putative signal tranduction protein Xp95; Signal transduction protein Xp95

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Gene Aliases: AI480591; AIP1; ALIX; AW544830; C76364; DRIP4; Eig2; HP95; KIAA1375; mKIAA1375; PDCD6IP; RGD1561176; XELAEV_18031317mg; xp95

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UniProt ID: (Human) Q8WUM4, (Rat) Q9QZA2, (Mouse) Q9WU78, (Xenopus) Q9W6C5

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Entrez Gene ID: (Human) 10015, (Rat) 501083, (Mouse) 18571, (Xenopus) 398095

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Function(s)

protein binding proteinase activated receptor binding protein homodimerization activity calcium-dependent protein binding SH3 domain binding protein dimerization activity

Process(es)

cell separation after cytokinesis apoptotic process nucleus organization mitotic metaphase plate congression regulation of centrosome duplication protein transport viral budding via host ESCRT complex cell division ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway regulation of mitotic spindle assembly positive regulation of exosomal secretion regulation of extracellular exosome assembly positive regulation of extracellular exosome assembly viral life cycle multivesicular body assembly transport cell cycle

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Alix Monoclonal Antibody (3A9)

Alix Antibody (MA1-83977) in ICC/IF

Product Details

MA1-83977

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Dot blot (DB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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