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Alpha-1-Antitrypsin Monoclonal Antibody (1A9G6)

Alpha-1-Antitrypsin Antibody in Immunocytochemistry (ICC/IF)

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Alpha-1-Antitrypsin Antibody (66135-1-IG) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 66135-1-IG (Alpha-1-Antitrypsin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Alpha-1-Antitrypsin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Alpha-1-Antitrypsin Antibody in Western Blot (WB)

Alpha-1-Antitrypsin Antibody in Flow Cytometry (Flow)

Alpha-1-Antitrypsin Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

66135-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1A9G6

Immunogen

Alpha-1-Antitrypsin Fusion Protein Ag9516 (28-418 aa encoded by BC015642)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.85 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: QGDAAQKTD TSHHDQDHPT FNKITPNLAE FAFSLYRQLA HQSNSTNIFF SPVSIATAFA MLSLGTKADT HDEILEGLNF NLTEIPEAQI HEGFQELLRT LNQPDSQLQL TTGNGLFLSE GLKLVDKFLE DVKKLYHSEA FTVNFGDTEE AKKQINDYVE KGTQGKIVDL VKELDRDTVF ALVNYIFFKG KWERPFEVKD TEEEDFHVDQ ATTVKVPMMK RLGMFNIQHC KKLSSWVLLM KYLGNATAIF FLPDEGKLQH LENELTHDII TKFLENEDRR SASLHLPKLS ITGTYDLKSV LGQLGITKVF SNGADLSGVT EEAPLKLSKA VHKAVLTIDE KGTEAAGAMF LEAIPMSIPP EVKFNKPFVF LMIEQNTKSP LFMGKVVNPT QK (28-418 aa encoded by BC015642)

Target Information

Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma.

Disease markers

Chen X,Liao X,Zheng B,Wang F,Chen F,Deng Z,Jiang H,Qin S

Tue Jan 31 00:00:00 UTC 2023

Separate roles of LMAN1 and MCFD2 in ER-to-Golgi trafficking of FV and FVIII.

Blood advances

Zhang Y,Liu Z,Zhang B

Tue Apr 11 00:00:00 UTC 2023

Novel Populations of Lung Capillary Endothelial Cells and Their Functional Significance.

Research square

James J,Dekan A,Niihori M,McClain N,Varghese M,Bharti D,Lawal OS,Padilla-Rodrigez M,Yi D,Dai Z,Gusev O,Rafikova O,Rafikov R

Wed May 03 00:00:00 UTC 2023

LMAN1-MCFD2 complex is a cargo receptor for the ER-Golgi transport of α1-antitrypsin.

The Biochemical journal

Zhang Y,Zhu M,Zheng C,Wei W,Emmer BT,Zhang B

Thu Apr 14 00:00:00 UTC 2022

Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro.

Annals of translational medicine

Luo S,Xiao S,Ai Y,Wang B,Wang Y

Mon Nov 01 00:00:00 UTC 2021

Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro.

Annals of translational medicine

Luo S,Xiao S,Ai Y,Wang B,Wang Y

Mon Nov 01 00:00:00 UTC 2021

Functional hit 1 (FH1)-based rapid and efficient generation of functional hepatocytes from human mesenchymal stem cells: a novel strategy for hepatic differentiation.

Annals of translational medicine

Luo S,Ai Y,Xiao S,Wang B,Wang Y

Thu Jul 01 00:00:00 UTC 2021

Functional hit 1 (FH1)-based rapid and efficient generation of functional hepatocytes from human mesenchymal stem cells: a novel strategy for hepatic differentiation.

Annals of translational medicine

Luo S,Ai Y,Xiao S,Wang B,Wang Y

Thu Jul 01 00:00:00 UTC 2021

Extensively expanded murine-induced hepatic stem cells maintain high-efficient hepatic differentiation potential for repopulation of injured livers.

Liver international : official journal of the International Association for the Study of the Liver

Yu B,Li H,Chen J,He Z,Sun H,Yang G,Shang C,Wang X,Li C,Chen Y,Hu Y

Tue Sep 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: a-1 AT; a1-antitrypsin; AAT; alpha 1 antitrypsin; alpha-1 antitrypsin; Alpha-1 protease inhibitor; Alpha-1 protease inhibitor 1; Alpha-1-antiproteinase; Alpha-1-antitrypsin; alpha-1-antitrypsin (protease inhibitor); Alpha-1-antitrypsin 1-1; alpha-1-antitrypsin 2; alpha-1-antitrypsin null; alpha-1-protease inhibitor; Alpha-1-proteinase inhibitor; Digestive Zymogen; MGC23330; MGC9222; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; Protease Serine 1; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1; serine (or cysteine) proteinase inhibitor, clade A, member 1; serine (or cysteine) proteinase inhibitor, clade A, member 1a; Serine Protease 1; Serine protease inhibitor 1-1; Serine protease inhibitor A1a; serine protease inhibitor alpha 1; Serpin A1; Serpin A1a; serpin peptidase inhibitor clade A member 1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1; TRP1; TRY1

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Gene Aliases: A1A; A1AT; AAT; Aat-2; Aat2; alpha1AT; Dom1; PI; PI1; PRO0684; PRO2209; PRO2275; SERPINA1; Serpina1a; Spi1; Spi1-1; Spi1-3

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UniProt ID: (Pig) P50447, (Human) P01009, (Rat) P17475, (Mouse) P07758

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Entrez Gene ID: (Pig) 397688, (Human) 5265, (Rat) 24648, (Mouse) 20700

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Function(s)

glycoprotein binding protease binding serine-type endopeptidase inhibitor activity protein binding identical protein binding endopeptidase inhibitor activity peptidase inhibitor activity

Process(es)

protein N-linked glycosylation acute-phase response negative regulation of peptidase activity negative regulation of endopeptidase activity response to cytokine response to peptide hormone platelet degranulation ER to Golgi vesicle-mediated transport blood coagulation COPII vesicle coating response to hypoxia inflammatory response response to inorganic substance response to lead ion response to organic cyclic compound response to estradiol response to lipopolysaccharide response to methanol response to triglyceride response to chromate negative regulation of serine-type endopeptidase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Alpha-1-Antitrypsin Monoclonal Antibody (1A9G6)

Alpha-1-Antitrypsin Antibody (66135-1-IG) in ICC/IF

Product Details

66135-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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