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Alpha Sarcoglycan Antibody (PA5-79990) in WB
Western blot was performed using Anti-Alpha Sarcoglycan Polyclonal Antibody (Product # PA5-79990) and a 43-55 kDa band corresponding to Alpha Sarcoglycan was observed across the tissues tested. Tissue extracts (30 µg lysate) of Mouse Kidney (Lane 1), Rat Kidney (Lane 2), Mouse Skeletal Muscle (Lane 3), Rat Skeletal Muscle (Lane 4), Mouse Heart (Lane 5) and Rat Heart (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # ... View More
Product Details
PA5-79990
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human SGCA recombinant protein (Position: Q24-H387).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747105
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Skeletal Muscle Tissue, Mouse Skeletal Muscle Tissue.
Target Information
The sarcoglycan transmembrane proteins are members of the dystrophin complex. Sarcoglycans cluster together to form a complex, which is localized in the cell membrane of skeletal, cardiac, and smooth muscle fibers. Four sarcoglycan subunit proteins, designated alpha-, beta-, gamma- and delta-sarcoglycan, form a complex on the skeletal muscle cell surface membrane. A genetic defect in any one of these proteins causes the loss or marked decrease of the whole sarcoglycan complex, which is observed in the autosomal recessive muscular dystrophy, sarcoglycanopathy. In smooth muscle, beta- and delta-sarcoglycans are associated with epsilon-sarcoglycan, a glycoprotein homologous to alpha-sarcoglycan. Additionally, a complete deficiency in delta-sarcoglycan is the cause of the Syrian hamster BIO.14 cardiomyopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 50 kDa dystrophin-associated glycoprotein; 50DAG; 50kD DAG; Adhalin; Alpha-sarcoglycan; Alpha-SG; Dystroglycan 2; Dystroglycan-2; Dystroglycan2; limb girdle muscular dystrophy 2D; sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein); SG alpha; SG-alpha
Gene Aliases: 50DAG; adhalin; ADL; Asg; DAG2; DMDA2; LGMD2D; SCARMD1; SGCA
UniProt ID: (Human) Q16586, (Mouse) P82350
Entrez Gene ID: (Human) 6442, (Rat) 303468, (Mouse) 20391
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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