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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Alpha-Smooth Muscle Actin Antibodies

          Invitrogen

          Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)), Biotin

          Advanced Verification
          2 Published Figures
          189 References
          View all (72) Alpha-Smooth Muscle Actin antibodies

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          Datasheet
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          Cite Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)), Biotin

          • Antibody Testing Data (6)
          • Published Figures (2)
          • Advanced Verification (1)
          Alpha-Smooth Muscle Actin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Alpha-Smooth Muscle Actin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          Alpha-Smooth Muscle Actin Antibody (MA5-11544) in WB

          Western blot analysis of Actin Sooth Muscle was performed by loading 25 µg of C2C12 (Lane 1), Hela (Lane 2), and A431 (Lane 3) cell lysates and a molecular weight protein ladder onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4ºC overnight. The membrane was probed with an Actin Sooth Muscle monoclonal antibody (Product # MA5-11547) at a dilu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Alpha-Smooth Muscle Actin Antibody in Western Blot (WB)
          Alpha-Smooth Muscle Actin Antibody in Western Blot (WB)
          Alpha-Smooth Muscle Actin Antibody in Immunocytochemistry (ICC/IF)
          Alpha-Smooth Muscle Actin Antibody in Immunocytochemistry (ICC/IF)
          Alpha-Smooth Muscle Actin Antibody in Immunocytochemistry (ICC/IF)
          Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (IHC)
          Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (IHC)
          Alpha-Smooth Muscle Actin Antibody

          Product Details

          MA5-11544

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:100-1:500
          View 16 publications 16 publications

          Immunohistochemistry (IHC)

          -
          View 142 publications 142 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:800
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          View 25 publications 25 publications

          Flow Cytometry (Flow)

          -
          View 3 publications 3 publications

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Bovine, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat

          Published species

          Cat, Dog, Human, Lizard, Mouse, Pig, Rabbit, Rat, Rhesus monkey, Sheep

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1A4 (asm-1)

          Immunogen

          N-terminal decapeptide of alpha-smooth muscle isoform of actin; acetylated at the N-terminus

          Conjugate

          Biotin Biotin Biotin
          • Unconjugated
          • Request custom conjugation

          Form

          Liquid

          Concentration

          40 µg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4, with 0.2% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10981631

          Product Specific Information

          MA5-11544 targets Actin Smooth Muscle in WB, immunofluorescence and immunohistochemistry applications and shows reactivity with cow, chicken, human, mouse, non-human primate, rabbit, and rat samples.

          The MA5-11544 immunogen is n-terminal decapeptide of alpha-smooth muscle isoform of actin; acetylated at the N-terminus.

          Target Information

          Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Actin Alpha 2 Smooth Muscle Aorta; Actin Vascular Smooth Muscle; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; alpha actin 2; Alpha Cardiac Actin; Alpha-actin; Alpha-actin-2; alpha-cardiac actin; alpha-smooth muscle actin; Aortic Smooth Muscle; asma; Cell growth-inhibiting gene 46 protein; Growth Inhibiting Gene 46; smooth muscle; smooth muscle alpha-actin

          View more View less

          Gene Aliases: 0610041G09Rik; a-SMA; AAT6; ACTA2; ACTSA; ACTVS; alphaSMA; asma; BOS_23286; GIG46; MYMY5; SMalphaA

          View more View less

          UniProt ID: (Rabbit) P62740, (Chicken) P08023, (Bovine) P62739, (Human) P62736, (Rat) P62738, (Mouse) P62737

          View more View less

          Entrez Gene ID: (Rabbit) 100009271, (Chicken) 423787, (Bovine) 515610, (Human) 59, (Rat) 81633, (Mouse) 11475

          View more View less

          Function(s)
          ATP binding protein kinase binding nucleotide binding protein binding
          Process(es)
          skeletal muscle thin filament assembly skeletal muscle fiber development muscle contraction regulation of blood pressure response to virus vascular smooth muscle contraction glomerular mesangial cell development positive regulation of gene expression mesenchyme migration
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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