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Invitrogen
Amphiphysin Polyclonal Antibody
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FIGURE: 1 / 7
Amphiphysin Antibody (PA5-21483) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Amphiphysin was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Amphiphysin Polyclonal Antibody (Product # PA5-21483) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-21483
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Amphiphysin. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.9 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152289
Product Specific Information
Recommended positive controls: mouse hippocampus, mouse brain, rat brain.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (91%), Pig (100%), Chicken (95%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein super family, play a key role in clathrin-mediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via its carboxy-terminal SH3 domain with dynamin, synaptojanin and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminal. Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amphiphysin; amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen); amphiphysin 1; amphiphysin I; HGNC:471
Gene Aliases: AMPH; AMPH1
UniProt ID: (Human) P49418, (Rat) O08838, (Mouse) Q7TQF7
Entrez Gene ID: (Human) 273, (Rat) 60668, (Mouse) 218038
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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