Invitrogen

Angiostatin Polyclonal Antibody

Angiostatin Antibody in Immunocytochemistry (ICC/IF)

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Angiostatin Antibody (PA1-600) in ICC/IF

Immunofluorescent analysis of Angiostatin (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Angiostatin polyclonal antibody (Product # PA1-600) in 3% BS... View More

Angiostatin Antibody in Western Blot (WB)

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Product Details

PA1-600

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: V(98) Y L S E C K T G I G N G Y R G T M S(116)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2167241

Product Specific Information

PA1-600 detects recombinant human and mouse angiostatin.

PA1-600 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a 50 kDa protein representing recombinant human angiostatin.

PA1-600 immunizing peptide corresponds to amino acid residues 98-116 from mouse angiostatin. This sequence is 95% and 84% conserved in rat and human angiostatin, respectively.

PA1-600 can be used with blocking peptide PEP-111.

Target Information

The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Janus Kinase 1 Plays a Critical Role in Mammary Cancer Progression.

Cell reports

Wehde BL,Rädler PD,Shrestha H,Johnson SJ,Triplett AA,Wagner KU

PA1-600 was used in Western Blotting to determine the role of Janus kinases in IL-6 cytokine signalling in metastatic cancer.

Tue Nov 20 00:00:00 UTC 2018

Treatment of medulloblastoma with oncolytic measles viruses expressing the angiogenesis inhibitors endostatin and angiostatin.

BMC cancer

Hutzen B,Bid HK,Houghton PJ,Pierson CR,Powell K,Bratasz A,Raffel C,Studebaker AW

PA1-600 was used in western blot to study the use of oncolytic measles viruses expressing endostatin and angiostatin for medulloblastoma therapy

Wed Mar 19 00:00:00 UTC 2014

Treatment of medulloblastoma with oncolytic measles viruses expressing the angiogenesis inhibitors endostatin and angiostatin.

BMC cancer

Hutzen B,Bid HK,Houghton PJ,Pierson CR,Powell K,Bratasz A,Raffel C,Studebaker AW

PA1-600 was used in western blot to study the use of oncolytic measles viruses expressing endostatin and angiostatin for medulloblastoma therapy

Wed Mar 19 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: angiostatin; Microplasmin; plasmin; plasmin heavy chain A; plasmin light chain B; Plasminogen

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Gene Aliases: AI649309; Pg; PLG

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UniProt ID: (Human) P00747, (Mouse) P20918

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Entrez Gene ID: (Human) 5340, (Mouse) 18815

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Function(s)

serine-type endopeptidase activity receptor binding protein binding serine-type peptidase activity protein domain specific binding apolipoprotein binding endopeptidase activity peptidase activity hydrolase activity

Process(es)

proteolysis blood coagulation hemostasis negative regulation of cell-substrate adhesion negative regulation of angiogenesis extracellular matrix disassembly tissue regeneration fibrinolysis myoblast differentiation muscle cell cellular homeostasis tissue remodeling proteolysis involved in cellular protein catabolic process negative regulation of fibrinolysis positive regulation of fibrinolysis trophoblast giant cell differentiation labyrinthine layer blood vessel development mononuclear cell migration platelet degranulation negative regulation of cell proliferation cellular protein metabolic process negative regulation of cell-cell adhesion mediated by cadherin

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Angiostatin Polyclonal Antibody

Angiostatin Antibody (PA1-600) in ICC/IF

Product Details

PA1-600

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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