Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Ankyrin 1 Monoclonal Antibody (N388A/10)
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 2
Ankyrin 1 Antibody (MA5-27723) in ICC/IF
Immunofluorescent analysis of Ankyrin 1 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Ankyrin 1 monoclonal antibody (Product # MA5-27723) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Image... View More
Product Details
MA5-27723
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
N388A/10
Immunogen
Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735111
Product Specific Information
A 1:100 dilution of MA5-27723 was sufficient for detection of Ankyrin R in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 200kDa. Does not cross-react with Ankyrin-B or Ankyrin-G.
This antibody was formerly sold as clone S388A-10.
Target Information
Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: ANK-1; ankyrin 1, erythrocytic; ankyrin 1, erythroid; Ankyrin-1; Ankyrin-R; Erythrocyte ankyrin; normoblastic anemia; rCG_43073
Gene Aliases: ANK; Ank-1; ANK1; nb; pale; SPH1; SPH2
UniProt ID: (Human) P16157, (Mouse) Q02357
Entrez Gene ID: (Human) 286, (Mouse) 11733, (Rat) 306570
exocytosis
ER to Golgi vesicle-mediated transport
cytoskeleton organization
signal transduction
positive regulation of organelle organization
maintenance of epithelial cell apical/basal polarity
protein targeting to plasma membrane
porphyrin-containing compound biosynthetic process
monovalent inorganic cation transport
erythrocyte development
iron ion homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support