Invitrogen

Ankyrin B Monoclonal Antibody (2.2)

3 Published Figures

7 References

View all (10) Ankyrin B antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (3)

Ankyrin B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

Ankyrin B Antibody (33-3700) in ICC/IF

Immunofluorescent analysis of Ankyrin B was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Ankyrin B Mouse monoclonal Antibody (Product # 33-3700) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperat... View More

Ankyrin B Antibody in Immunohistochemistry (IHC)

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Product Details

33-3700

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:500

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fruit fly, Hamster, Insect, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2.2

Immunogen

Synthetic peptide derived from the human ankyrin B protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533115

Target Information

This gene encodes a member of the ankyrin family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton. Ankyrins play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. The protein encoded by this gene is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations in this gene cause long QT syndrome 4. Multiple transcript variants encoding different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Cantú syndrome mutation produces dual effects on KATP channels by disrupting ankyrin B regulation.

The Journal of general physiology

Crespo-García T,Rubio-Alarcón M,Cámara-Checa A,Dago M,Rapún J,Nieto-Marín P,Marín M,Cebrián J,Tamargo J,Delpón E,Caballero R

Published figure using Ankyrin B monoclonal antibody (Product # 33-3700) in Western Blot

Mon Jan 02 00:00:00 UTC 2023

The L1 cell adhesion molecule constrains dendritic spine density in pyramidal neurons of the mouse cerebral cortex.

Frontiers in neuroanatomy

Murphy KE,Wade SD,Sperringer JE,Mohan V,Duncan BW,Zhang EY,Pak Y,Lutz D,Schachner M,Maness PF

33-3700 was used in Western Blotting to provide insight into the molecular mechanism of spine regulation and underscores the potential for this adhesion molecule to regulate cognitive and other L1-related functions that are abnormal in the L1 syndrome.

Sat May 13 00:00:00 UTC 2023

The L1 cell adhesion molecule constrains dendritic spine density in pyramidal neurons of the mouse cerebral cortex.

Frontiers in neuroanatomy

Murphy KE,Wade SD,Sperringer JE,Mohan V,Duncan BW,Zhang EY,Pak Y,Lutz D,Schachner M,Maness PF

Sat May 13 00:00:00 UTC 2023

PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B.

Nature communications

Bock T,Türk C,Aravamudhan S,Keufgens L,Bloch W,Rozsivalova DH,Romanello V,Nogara L,Blaauw B,Trifunovic A,Braun T,Krüger M

33-3700 was used in Immunohistochemistry to demonstrate if PERM1 interacts with the intracellular adaptor protein ankyrin B that connects the cytoskeleton to the plasma membrane.

Thu Aug 12 00:00:00 UTC 2021

PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B.

Nature communications

Bock T,Türk C,Aravamudhan S,Keufgens L,Bloch W,Rozsivalova DH,Romanello V,Nogara L,Blaauw B,Trifunovic A,Braun T,Krüger M

33-3700 was used in Immunohistochemistry to demonstrate if PERM1 interacts with the intracellular adaptor protein ankyrin B that connects the cytoskeleton to the plasma membrane.

Thu Aug 12 00:00:00 UTC 2021

Coupling of the Na+/K+-ATPase to Ankyrin B controls Na+/Ca2+ exchanger activity in cardiomyocytes.

Cardiovascular research

Skogestad J,Aronsen JM,Tovsrud N,Wanichawan P,Hougen K,Stokke MK,Carlson CR,Sjaastad I,Sejersted OM,Swift F

33-3700 was used in Western Blot to investigate the role of NKA binding to AnkB in cardiomyocytes.

Wed Jan 01 00:00:00 UTC 2020

Activation of glial FGFRs is essential in glial migration, proliferation, and survival and in glia-neuron signaling during olfactory system development.

PloS one

Gibson NJ,Tolbert LP,Oland LA

33-3700 was used in immunocytochemistry to investigate the role of Fibroblast Growth Factor receptors in glial migration, proliferation, and survival.

Tue Sep 04 00:00:00 UTC 2012

Roles of specific membrane lipid domains in EGF receptor activation and cell adhesion molecule stabilization in a developing olfactory system.

PloS one

Gibson NJ,Tolbert LP,Oland LA

33-3700 was used in immunohistochemistry - paraffin section to review factors that contribute to the development of the olfactory system of Manduca sexta

Tue Sep 29 00:00:00 UTC 2009

Structural requirements for interaction of sodium channel beta 1 subunits with ankyrin.

The Journal of biological chemistry

Malhotra JD,Koopmann MC,Kazen-Gillespie KA,Fettman N,Hortsch M,Isom LL

Fri Jul 19 00:00:00 UTC 2002

Structural requirements for interaction of sodium channel beta 1 subunits with ankyrin.

The Journal of biological chemistry

Malhotra JD,Koopmann MC,Kazen-Gillespie KA,Fettman N,Hortsch M,Isom LL

Fri Jul 19 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: ANK-2; ank2; ankyrin 2, brain; ankyrin 3, epithelial; ankyrin B; ankyrin, brain; Ankyrin-2; ankyrin-2, nonerythrocytic; Ankyrin-B; Brain ankyrin; Non-erythroid ankyrin

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Gene Aliases: AI835472; ANK-2; ANK2; Ank3; ANKB; AW491075; brank-2; LQT4

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UniProt ID: (Human) Q01484, (Mouse) Q8C8R3

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Entrez Gene ID: (Human) 287, (Mouse) 109676, (Rat) 362036

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Function(s)

structural constituent of cytoskeleton protein binding cytoskeletal adaptor activity enzyme binding protein kinase binding spectrin binding protein binding, bridging ion channel binding ATPase binding potassium channel regulator activity

Process(es)

regulation of heart rate cellular calcium ion homeostasis signal transduction protein localization positive regulation of gene expression regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion regulation of cardiac muscle contraction by calcium ion signaling regulation of protein stability T-tubule organization protein localization to cell surface cellular protein localization protein localization to M-band protein localization to T-tubule regulation of apoptotic process positive regulation of potassium ion transport protein stabilization regulation of calcium ion transport positive regulation of calcium ion transport regulation of cardiac muscle contraction cardiac muscle contraction protein localization to endoplasmic reticulum protein localization to plasma membrane protein targeting to plasma membrane regulation of cardiac muscle cell contraction atrial cardiac muscle cell action potential SA node cell action potential regulation of cardiac muscle cell membrane potential atrial cardiac muscle cell to AV node cell communication SA node cell to atrial cardiac muscle cell communication regulation of heart rate by cardiac conduction regulation of AV node cell action potential regulation of SA node cell action potential regulation of atrial cardiac muscle cell action potential positive regulation of potassium ion transmembrane transporter activity regulation of calcium ion transmembrane transporter activity positive regulation of calcium ion transmembrane transporter activity regulation of cation channel activity positive regulation of cation channel activity atrial septum development ER to Golgi vesicle-mediated transport cytoskeleton organization paranodal junction assembly protein localization to organelle regulation of release of sequestered calcium ion into cytosol response to methylmercury regulation of ventricular cardiac muscle cell membrane repolarization sarcoplasmic reticulum calcium ion transport ventricular cardiac muscle cell action potential membrane depolarization during SA node cell action potential nervous system development

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ankyrin B Monoclonal Antibody (2.2)

Ankyrin B Antibody (33-3700) in ICC/IF

Product Details

33-3700

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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